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NHP2L1 antibody [N1C3]

Anti-NHP2L1 antibody [N1C3] used in IHC (Whole mount) (IHC-Wm). GTX109247
Anti-NHP2L1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX109247
Anti-NHP2L1 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109247
Anti-NHP2L1 antibody [N1C3] used in Western Blot (WB). GTX109247
Anti-NHP2L1 antibody [N1C3] used in Western Blot (WB). GTX109247
Anti-NHP2L1 antibody [N1C3] used in Western Blot (WB). GTX109247

Cat. No. GTX109247

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Wm

Reactivity

Human, Mouse, Rat, Zebrafish
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Wm Assay dependent
Not tested in other applications.

Calculated MW

14 kDa. ( Note )

Positive Control

HeLa , HepG2 , PC-12 , Rat2 , Neuro2A , GL261 , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12

Predict Reactivity

Bovine, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human NHP2L1 Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950997

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

small nuclear ribonucleoprotein 13 , 15.5K , FA-1 , FA1 , NHP2L1 , NHPX , OTK27 , SNRNP15-5 , SPAG12 , SSFA1

Cellular Localization

Nucleus , nucleolus

Background

Originally named because of its sequence similarity to the Saccharomyces cerevisiae NHP2 (non-histone protein 2), this protein appears to be a highly conserved nuclear protein that is a component of the [U4/U6.U5] tri-snRNP. It binds to the 5' stem-loop of U4 snRNA. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-NHP2L1 antibody [N1C3] used in IHC (Whole mount) (IHC-Wm). GTX109247

GTX109247 IHC-Wm Image

NHP2L1 antibody [N1C3] detects Nhp2l1 protein at nucleoli on whole-mount zebrafish embryos by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed zebrafish embryos.
NHP2L1 antibody [N1C3] (GTX109247) dilution: 1:200.

Anti-NHP2L1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX109247

GTX109247 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using NHP2-like protein 1(GTX109247) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-NHP2L1 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109247

GTX109247 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NHP2-like protein 1(GTX109247) antibody at 1:200 dilution.

Anti-NHP2L1 antibody [N1C3] used in Western Blot (WB). GTX109247

GTX109247 WB Image

NHP2L1 antibody detects NHP2L1 protein by Western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat2 whole cell lysate/extract
15 % SDS-PAGE
NHP2L1 antibody (GTX109247) dilution: 1:1000

Anti-NHP2L1 antibody [N1C3] used in Western Blot (WB). GTX109247

GTX109247 WB Image

Sample (30 ug of whole cell lysate)
A: HeLa
B: Hep G2 (GTX27900)
12% SDS PAGE
GTX109247 diluted at 1:1000

Anti-NHP2L1 antibody [N1C3] used in Western Blot (WB). GTX109247

GTX109247 WB Image

NHP2L1 antibody detects NHP2L1 protein by Western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg GL261 whole cell lysate/extract
C. 30 μg C8D30 whole cell lysate/extract
D. 30 μg NIH-3T3 whole cell lysate/extract
E. 30 μg BCL-1 whole cell lysate/extract
F. 30 μg Raw264.7 whole cell lysate/extract
G. 30 μg C2C12 whole cell lysate/extract
15 % SDS-PAGE
NHP2L1 antibody (GTX109247) dilution: 1:1000

REFERENCE

There are currently no references for NHP2L1 antibody [N1C3] (GTX109247). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for NHP2L1 antibody [N1C3] (GTX109247). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169