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Leptin antibody

Anti-Leptin antibody used in Western Blot (WB). GTX22095

Cat. No. GTX22095

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ELISA, IHC

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all Leptin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
ELISA 1:5000-1:20000
IHC Assay dependent
Not tested in other applications.

Calculated MW

19 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Recombinant mouse leptin 16,000 MW produced in E. coli.

Purification

IgG fraction
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

leptin , ob , obese

Cellular Localization

Secreted

Background

Obesity associated leptin is the product of the OB gene and has been identified with Type II diabetes.

Database

Research Area

DATA IMAGES

Anti-Leptin antibody used in Western Blot (WB). GTX22095

GTX22095 WB Image

WB analysis of recombinant mouse leptin protein using GTX22095 Leptin antibody.
Lane 1 : Reduced recombinant mouse leptin
Lane 2 : Protein ladder
Lane 3 : Non-reduced recombinant mouse leptin
Loading : 0.03 μg
Dilution : 1:1000

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399