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Gata1a antibody

Anti-Gata1a antibody used in IHC (Whole mount) (IHC-Wm). GTX128333
Anti-Gata1a antibody used in Western Blot (WB). GTX128333

Cat. No. GTX128333

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-Wm

Reactivity

 Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all zebrafish Gata1a products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-Wm 1:100-1:500
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Zebrafish

Immunogen

Recombinant protein encompassing a sequence within the center region of zebrafish Gata1a. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885757

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

GATA binding protein 1a , gata1 , gta1 , sb:eu551 , vlt , zg1

Database

Research Area

DATA IMAGES

Anti-Gata1a antibody used in IHC (Whole mount) (IHC-Wm). GTX128333

GTX128333 IHC-Wm Image

Gata1a antibody detects Gata1a protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 24 hours-post-fertilization zebrafish embryo.
Gata1a antibody (GTX128333) dilution: 1:100.
Anti-Gata1a antibody used in Western Blot (WB). GTX128333

GTX128333 WB Image

Zebrafish tissue extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Gata1a antibody (GTX128333) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Chen C et al. Cell Death Dis 2020; 11 (2) : 135 The nuclear gene rpl18 regulates erythroid maturation via JAK2-STAT3 signaling in zebrafish model of Diamond-Blackfan anemia.
Application : WB
Reactivity : Zebrafish
Tyrkalska SD et al. Immunity 2019; (Epub) Inflammasome Regulates Hematopoiesis through Cleavage of the Master Erythroid Transcription Factor GATA1.
Application : WB
Reactivity : Zebrafish
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169