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GSTZ1 antibody [N1C3]

KO/KD Orthogonal Overexpression
Anti-GSTZ1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX106109
Anti-GSTZ1 antibody [N1C3] used in Western Blot (WB). GTX106109
Anti-GSTZ1 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106109
Anti-GSTZ1 antibody [N1C3] used in Western Blot (WB). GTX106109
Anti-GSTZ1 antibody [N1C3] used in Western Blot (WB). GTX106109
Anti-GSTZ1 antibody [N1C3] used in Western Blot (WB). GTX106109

Cat. No. GTX106109

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all GSTZ1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

Positive Control

293T

Predict Reactivity

Mouse, Rat, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.98 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GSTZ1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037105

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glutathione S-transferase zeta 1 , GSTZ1-1 , MAAI , MAAID , MAI

Cellular Localization

Cytoplasm

Background

This gene is a member of the glutathione S-transferase (GSTs) super-family which encodes multifunctional enzymes important in the detoxification of electrophilic molecules, including carcinogens, mutagens, and several therapeutic drugs, by conjugation with glutathione. This enzyme also plays a significant role in the catabolism of phenylalanine and tyrosine. Thus defects in this enzyme may lead to severe metabolic disorders including alkaptonuria, phenylketonuria and tyrosinaemia. Several transcript variants of this gene encode multiple protein isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GSTZ1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX106109

GTX106109 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using MAAI(GTX106109) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GSTZ1 antibody [N1C3] used in Western Blot (WB). GTX106109

GTX106109 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
12% SDS PAGE
GTX106109 diluted at 1:1000

Anti-GSTZ1 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106109

GTX106109 ICC/IF Image

GSTZ1 antibody [N1C3] detects GSTZ1 protein at cytoplasm by immunofluorescent analysis.
Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min.
Green: GSTZ1 protein stained by GSTZ1 antibody [N1C3] (GTX106109) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-GSTZ1 antibody [N1C3] used in Western Blot (WB). GTX106109

GTX106109 WB Image

KO/KD Overexpression

The data was published in the journal FEBS Open Bio in 2020. PMID: 31782257

Anti-GSTZ1 antibody [N1C3] used in Western Blot (WB). GTX106109

GTX106109 WB Image

Orthogonal

The data was published in the journal J Exp Clin Cancer Res in 2019. PMID: 31666108

Anti-GSTZ1 antibody [N1C3] used in Western Blot (WB). GTX106109

GTX106109 WB Image

KO/KD Overexpression

The data was published in the journal FEBS Open Bio in 2020. PMID: 31782257

REFERENCE

Application Reference

Luo H et al. JCI Insight 2023; 8 (23) Signaling metabolite succinylacetone activates HIF-1α and promotes angiogenesis in GSTZ1-deficient hepatocellular carcinoma.
Qiujie Wang et al. Cell Death Dis 2021; 12 (5) : 426 GSTZ1 sensitizes hepatocellular carcinoma cells to sorafenib-induced ferroptosis via inhibition of NRF2/GPX4 axis.
Application : WB
Reactivity : Human
Lei C et al. FEBS Open Bio 2020; 10 (1) : 6月17日 GSTZ1-1 downregulates Wnt/β-catenin signalling in hepatocellular carcinoma cells.
Li J et al. J Exp Clin Cancer Res 2019; 38 (1) : 438 GSTZ1 deficiency promotes hepatocellular carcinoma proliferation via activation of the KEAP1/NRF2 pathway.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169