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PATJ antibody [N1], N-term

Anti-PATJ antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX104753
Anti-PATJ antibody [N1], N-term used in Western Blot (WB). GTX104753

Cat. No. GTX104753

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all PATJ products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

196 kDa. ( Note )

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human PATJ. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1241157

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

PATJ crumbs cell polarity complex component , Cipp , INADL , InaD-like , hINADL

Cellular Localization

Membrane , Cell junction , tight junction , Apical cell membrane

Background

This gene encodes a protein with multiple PDZ domains. PDZ domains mediate protein-protein interactions, and proteins with multiple PDZ domains often organize multimeric complexes at the plasma membrane. This protein localizes to tight junctions and to the apical membrane of epithelial cells. A similar protein in Drosophila is a scaffolding protein which tethers several members of a multimeric signaling complex in photoreceptors. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PATJ antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX104753

GTX104753 IHC-P Image

PATJ antibody [N1], N-term detects PATJ protein at cytoplasm in human colon by immunohistochemical analysis.
Sample: Paraffin-embedded human colon.
PATJ antibody [N1], N-term (GTX104753) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PATJ antibody [N1], N-term used in Western Blot (WB). GTX104753

GTX104753 WB Image

Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with PATJ antibody [N1], N-term (GTX104753) diluted at 1:500.

REFERENCE

Application Reference

Gagnoux-Palacios L et al. J Cell Biol 2018; (Epub) Cell polarity and adherens junction formation inhibit epithelial Fas cell death receptor signaling.
Application : WB
Reactivity : Human
Jiang X et al. Gene Expr Patterns 2017; 27 : 93-98 Asymmetric distribution of CRUMBS polarity complex proteins from compacted 8-cell to blastocyst stage during mouse preimplantation development.
Lennon FE et al. J Biol Chem 2014; 289 (35) : 24043-58 Transactivation of the receptor-tyrosine kinase ephrin receptor A2 is required for the low molecular weight hyaluronan-mediated angiogenesis that is implicated in tumor progression.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169