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Human DPH4 protein, His tag

Human DPH4 protein, His tag. GTX57310-pro

Cat. No. GTX57310-pro

Species

 Human
Package
50 μg ($389)
See all DPH4 protein products

APPLICATION

Observed MW

17 kDa.

PROPERTIES

Form

Liquid

Buffer

20mM Tris-HCl, 150mM NaCl, 10% Glycerol, 1mM DTT

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Region/Sequence

Full length protein, N-terminal His-Tag; MGSSHHHHHH SSGLVPRGSH MGSMMAVEQM PKKDWYSILG ADPSANISDL KQKYQKLILM YHPDKQSTDV PAGTVEECVQ KFIEIDQAWK ILGNEETKRE YDLQRCEDDL RNVGPVDAQV YLEEMSWNEG DHSFYLSCRC GGKYSVSKDE AEEVSLISCD TCSLIIELLH YN

Expression System

E. coli

Purity

> 90% by SDS-PAGE.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DnaJ heat shock protein family (Hsp40) member C24 , DPH4 , JJJ3 , ZCSL3

Cellular Localization

Cytoplasm, cytoskeleton

Background

Diphthamide is a unique posttranslationally modified histidine found only in translation elongation factor-2 (EEF2; MIM 130610). This modification is conserved from archaebacteria to humans and serves as the target for ADP-ribosylation and inactivation of EEF2 by diphtheria toxin (DT) and Pseudomonas exotoxin A. DPH4 is 1 of several enzymes involved in synthesis of diphthamide in EEF2 (Liu et al., 2004 [PubMed 15485916]).[supplied by OMIM, Mar 2008]

Database

Research Area

DATA IMAGES

Human DPH4 protein, His tag. GTX57310-pro

GTX57310-pro Image

3μg Human DPH4 protein (GTX57310-pro) by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

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SDS
Glycerol.pdf
Package List Price ($)
$ 389