GeneTex's KO-Validated CD44 Recombinant Monoclonal Antibody

03-Aug-2023

Cluster of Differentiation 44 (CD44) is a non-kinase cell surface transmembrane glycoprotein that is the major receptor for the extracellular matrix component hyaluronate. It exists as the smallest and most expressed CD44 standard (CD44s) isoform as well as a range of variant (CD44v) isoforms generated by alternative splicing. In addition to functioning in various aspects of hemopoiesis, angiogenesis, and cytokine release, CD44 proteins are overexpressed in cancer stem cells and interact with intracellular signaling networks to support the oncogenic properties of tumor cells and drive cancer cell progression, metastasis, and therapy resistance (1).

GeneTex is proud to offer a newly released knockout (KO)-validated recombinant rabbit monoclonal CD44 antibody [HL1650] (GTX637231) to detect CD44 in several applications. This antibody also recognizes both the CD44s and CD44v isoforms by western blot. Please see the product images below and visit www.genetex.com for more details.

CD44 antibody [HL1650] (GTX637231)

Detection of CD44v and CD44s proteins in various human cell lysates.

Detection of glycosylated and de-glycosylated CD44 proteins in Mock or Tunicamycin-treated MDA-MB-231 cell lysates.

HeLa cell wildtype (WT) and CD44-knockout (KO) lysates demonstrate antibody specificity.

WB analysis of A549 cell and LNCaP cell lysates display differential CD44 expression and more robust performance of GeneTex’s antibody compared to a competitor’s reagent.

ICC/IF staining of MeOH-fixed A431 cells demonstrating expected CD44 expression on the cell membrane.

Detection of CD44 in paraffin-embedded human ovarian cancer tissue.

Reference:

Biomolecules. 2021 Dec; 11(12): 1850.

GeneTex's KO-Validated CD44 Recombinant Monoclonal Antibody

CD44 antibody [HL1650] (GTX637231)

Detection of CD44v and CD44s proteins in various human cell lysates.

Detection of glycosylated and de-glycosylated CD44 proteins in Mock or Tunicamycin-treated MDA-MB-231 cell lysates.

HeLa cell wildtype (WT) and CD44-knockout (KO) lysates demonstrate antibody specificity.

WB analysis of A549 cell and LNCaP cell lysates display differential CD44 expression and more robust performance of GeneTex’s antibody compared to a competitor’s reagent.

ICC/IF staining of MeOH-fixed A431 cells demonstrating expected CD44 expression on the cell membrane.

Detection of CD44 in paraffin-embedded human ovarian cancer tissue.

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