APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:5,000 |
1:20-100 |
Assay dependent |
1:1,000 |
Assay dependent |
Assay dependent |
Not tested in other applications.
Product Note
This antibody detects nicotinic acetylcholine receptor (nAChR) gamma and delta subunits. This antibody does not detect the alpha 1 and beta 1 subunits.
PROPERTIES
Form
Liquid
Buffer
Ascites
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Immunogen
Purified Torpedo californica acetylcholine receptor.
Purification
Unpurified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
cholinergic receptor nicotinic delta subunit , cholinergic receptor nicotinic gamma subunit , ACHRD , CMS2A , CMS3A , CMS3B , CMS3C , FCCMS , SCCMS , ACHRG
Cellular Localization
Cell membrane; Multi-pass membrane protein
Background
Acetylcholine receptors (AChRs) mediate synaptic transmission at the neuromuscular junction. The channel-linked AChR that mediates rapid, excitatory actions of acetylcholine is called nicotinic AChR (nAChR) because it can be activated by nicotine. The non-channel linked AChR that medicates the slow actions of acetylcholine, which can be either inhibitory or excitatory, is called muscarinic AChR (mAChR) because it can be activated by muscarine. The nAChRs fall into two subclasses, the muscle-type and the neuronal-type. The muscle-type receptor is a pentameric glycoprotein of five membrane spanning subunits, (2 alpha 1’s, beta 1, gamma or epsilon and delta) which form a ligand gated ion channel. The neuronal nAChR is also a pentamer, but unlike the muscle-type, is made up of only two general types of subunits, alpha and beta of which multiple subtypes have been described (alpha 2 to alpha 8 and beta 2 to beta 4).
Database
Research Area
DATA IMAGES
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GTX22804 IHC-P Image
IHC-P analysis of mouse kidney tissue using GTX22804 AChR antibody [88B]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:20
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GTX22804 ICC/IF Image
ICC/IF analysis of formaldehyde fixed U251 cells using GTX22804 AChR antibody [88B]. Cells were probed without (right) or with (left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Dilution : 1:100 overnight at 4 oC
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GTX22804 ICC/IF Image
ICC/IF analysis of formaldehyde fixed Neuro-2a cells using GTX22804 AChR antibody [88B]. Cells were probed without (right) or with (left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Dilution : 1:100 overnight at 4 oC
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GTX22804 ICC/IF Image
ICC/IF analysis of formaldehyde fixed HeLa cells using GTX22804 AChR antibody [88B]. Cells were probed without (right) or with (left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Dilution : 1:100 overnight at 4 oC
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GTX22804 IHC-P Image
IHC-P analysis of mouse lymph node tissue using GTX22804 AChR antibody [88B]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:20
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GTX22804 IHC-P Image
IHC-P analysis of mouse brain tissue using GTX22804 AChR antibody [88B]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:20
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REFERENCE
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REVIEW
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