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BCKDK antibody [11C9]

Anti-BCKDK antibody [11C9] used in IHC (Paraffin sections) (IHC-P). GTX84836
Anti-BCKDK antibody [11C9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84836
Anti-BCKDK antibody [11C9] used in Western Blot (WB). GTX84836
Anti-BCKDK antibody [11C9] used in IHC (Paraffin sections) (IHC-P). GTX84836

Cat. No. GTX84836

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 11C9

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($519)
See all BCKDK products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF 1:100
IHC-P 1:50
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.8 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human BCKDK (NP_005872) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

branched chain ketoacid dehydrogenase kinase , BCKDKD , BDK

Cellular Localization

Mitochondrion matrix,Mitochondrion

Background

Catalyzes the phosphorylation and inactivation of the branched-chain alpha-ketoacid dehydrogenase complex, the key regulatory enzyme of the valine, leucine and isoleucine catabolic pathways. Key enzyme that regulate the activity state of the BCKD complex.

Database

Research Area

DATA IMAGES

Anti-BCKDK antibody [11C9] used in IHC (Paraffin sections) (IHC-P). GTX84836

GTX84836 IHC-P Image

IHC-P analysis of human endometrium tissue using GTX84836 BCKDK antibody [11C9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-BCKDK antibody [11C9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84836

GTX84836 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with BCKDK plasmid using GTX84836 BCKDK antibody [11C9].

Anti-BCKDK antibody [11C9] used in Western Blot (WB). GTX84836

GTX84836 WB Image

WB analysis of HEK293T cells transfected with BCKDK plasmid (Right) or empty vector (Left) for 48 hrs using GTX84836 BCKDK antibody [11C9].
Loading : 5 ug per lane

Anti-BCKDK antibody [11C9] used in IHC (Paraffin sections) (IHC-P). GTX84836

GTX84836 IHC-P Image

IHC-P analysis of human ovary tissue using GTX84836 BCKDK antibody [11C9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519