GeneTex
United States (US)

Bax antibody [N1N2], N-term

GTX109683_39988_IP_19011507_672.jpg
GTX109683_39988_20141205_IHC_19011507_779.jpg
GTX109683_39988_20160525_IFA_19011507_473.jpg
GTX109683_WB_19011507_320.jpg

Cat No. GTX109683

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Positive Control

293T , HeLaS3 , HepG2 , Molt-4 , Raji , *U2OS ,

Predict Reactivity

Bovine, Dog, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Bax. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

BCL2L4 antibody, BAX antibody, bcl-2-like protein 4 antibody, apoptosis regulator BAX antibody, bcl2-L-4 antibody, BCL2-associated X protein antibody

Cellular Localization

Isoform Alpha: Mitochondrion membrane , Cytoplasm , Isoform Beta: Cytoplasm , Isoform Gamma: Cytoplasm , Isoform Delta: Cytoplasm

Background

The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX109683_39988_IP_19011507_672.jpg

GTX109683 IP Image

Bax antibody [N1N2], N-term immunoprecipitates BCL2-associated X protein in IP experiments.
IP samples: MCF-7 whole cell extract
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of BCL2-associated X protein by 4 μg Bax antibody [N1N2], N-term (GTX109683)
15 % SDS-PAGE
The immunoprecipitated BCL2-associated X protein was detected by Bax antibody [N1N2], N-term (GTX109683) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

GTX109683_39988_20141205_IHC_19011507_779.jpg

GTX109683 IHC-P Image

Bax antibody [N1N2], N-term detects Bax protein at cytoplasm on human lung carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung carcinoma.
Bax antibody [N1N2], N-term (GTX109683) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX109683_39988_20160525_IFA_19011507_473.jpg

GTX109683 ICC/IF Image

Bax antibody [N1N2], N-term detects Bax protein at cytoplasm, nucleus and mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Bax protein stained by Bax antibody [N1N2], N-term (GTX109683) diluted at 1:400.
Blue: Hoechst 33342 staining.

GTX109683_WB_19011507_320.jpg

GTX109683 WB Image

Sample(30 µg whole cell lysate)
A:Hep G2 (GTX27900)
12% SDS PAGE
GTX109683 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW

Bax antibody [N1N2], N-term Cat No. GTX109683

Rating ( Average 5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 5 based on 2 users reviews)
Date : Anonymous submitted on 26-May-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : MG63 cells
Amount used : 30 μg
Blocking : 5% non-fat dry milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, overnightHr
Lane Description : Lane 1: Untreated MG63 whole cell lysate, Lane 2: Treated (IOX-1; 3uM) MG63 whole cell lysate, Lane 3: Treated (IOX-1; 10uM) MG63 whole cell lysateA:Untreated MG63 whole cell lysate
Date : Anonymous submitted on 15-Jan-2013
Score :
Application Tested : WB
Sample Species : Hu
Sample : U2OS cells
Amount used : 30μg
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:2000, 4°C, overnightHr
Application Reference
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169