APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500 |
1:500 |
1:200 |
1:100 |
Assay dependent |
Assay dependent |
Assay dependent |
Not tested in other applications.
Calculated MW
Product Note
This antibody detects one or more immunologically related proteins in mouse cell line Heb7a that does not contain CFTR mRNA.
PROPERTIES
Form
Liquid
Buffer
Ascites
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Antigen Species
Human
Immunogen
Synthetic Peptide: G(103) R I I A S Y D P D N K E E R(117)
Purification
Unpurified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
cystic fibrosis transmembrane conductance regulator , ABC35 , ABCC7 , CF , CFTR/MRP , MRP7 , TNR-CFTR , dJ760C5.1
Cellular Localization
Apical cell membrane,Cell membrane,Endoplasmic reticulum membrane,Nucleus
Background
This gene encodes a member of the ATP-binding cassette (ABC) transporter superfamily. The encoded protein functions as a chloride channel, making it unique among members of this protein family, and controls ion and water secretion and absorption in epithelial tissues. Channel activation is mediated by cycles of regulatory domain phosphorylation, ATP-binding by the nucleotide-binding domains, and ATP hydrolysis. Mutations in this gene cause cystic fibrosis, the most common lethal genetic disorder in populations of Northern European descent. The most frequently occurring mutation in cystic fibrosis, DeltaF508, results in impaired folding and trafficking of the encoded protein. Multiple pseudogenes have been identified in the human genome. [provided by RefSeq, Aug 2017]
Database
Research Area
DATA IMAGES
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GTX22784 IHC-P Image
IHC-P analysis of human colon carcinoma tissue using GTX22784 CFTR antibody [CF3]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200
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GTX22784 IHC-P Image
IHC-P analysis of human pancreas tissue using GTX22784 CFTR antibody [CF3]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200
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GTX22784 ICC/IF Image
ICC/IF analysis of WiDr cells using GTX22784 CFTR antibody [CF3]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4ºC
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GTX22784 IHC-P Image
IHC-P analysis of human tonsil tissue using GTX22784 CFTR antibody [CF3]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200
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GTX22784 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX22784 CFTR antibody [CF3]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4ºC
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GTX22784 ICC/IF Image
ICC/IF analysis of U251 cells using GTX22784 CFTR antibody [CF3]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4ºC
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REFERENCE
There are currently no references for CFTR antibody [CF3] (GTX22784). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for CFTR antibody [CF3] (GTX22784). Be the first to share your experience with this product.