GeneTex
United States (US)

Cardiac Troponin I antibody [10B11]

Cardiac-Troponin-I-antibody-10B11_Western blot_GTX10225-1_19011720_229.jpg

Cat No. GTX10225

Host Mouse
Clonality Monoclonal
Clone Name 10B11
Isotype IgG1
Application WB, Apuri, IA
Reactivity Human, Rat, Rabbit, Cat, Dog, Pig
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
Apuri Assay dependent
IA Assay dependent
Not tested in other applications.

Calculated MW

24.01 kDa. ( Note )

Specificity/Sensitivity

This antibody reacts with free cardiac troponin I (cTnI) and cTnI forming complexes with other troponin components (in the presence of 5 mM EDTA). Not affected by heparin, phosphorylation, oxidation and troponin complex formation. Recognizes dephospho- and phospho- forms of cardiac Troponin I. Does not cross-react with skeletal muscle troponin I.
PROPERTIES

Form

Liquid

Buffer

PBS pH 7.4, 0.09 % sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4º C.

Concentration

Batch dependent(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Free human cardiac troponin I, native human cardiac troponin complex, or human cardiac troponin I peptides.

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Troponin I3, Cardiac Type,Cmd1Ff,Cmd2A,Cmh7,Rcm1,Tnnc1,Ctni,Tnni3

Background

Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Cardiac-Troponin-I-antibody-10B11_Western blot_GTX10225-1_19011720_229.jpg

GTX10225 WB Image

Recognition of the dephospho- and phospho- forms of human cardiac troponin I. A. Cardiac Troponin I antibody [10B11] (GTX10225); B. Cardiac Troponin I antibody [8E10] (GTX10232)

Package List Price ($)
$ 289