GeneTex

  • Country / Location Selection

United States (US)

Cyclophilin F antibody [N1C3]

KO/KD Orthogonal
Anti-Cyclophilin F antibody [N1C3] used in Western Blot (WB). GTX117951
Anti-Cyclophilin F antibody [N1C3] used in Western Blot (WB). GTX117951
Anti-Cyclophilin F antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX117951
Anti-Cyclophilin F antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117951
Anti-Cyclophilin F antibody [N1C3] used in Western Blot (WB). GTX117951
Anti-Cyclophilin F antibody [N1C3] used in Western Blot (WB). GTX117951

Cat. No. GTX117951

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Cyclophilin F products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Positive Control

rat heart , mouse heart , A431 , 293T

Predict Reactivity

Bovine, Chicken, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.67 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cyclophilin F. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11170163

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

peptidylprolyl isomerase F , CYP3 , CyP-M , Cyp-D , CypD

Cellular Localization

Mitochondrion matrix

Background

The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein is part of the mitochondrial permeability transition pore in the inner mitochondrial membrane. Activation of this pore is thought to be involved in the induction of apoptotic and necrotic cell death. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Cyclophilin F antibody [N1C3] used in Western Blot (WB). GTX117951

GTX117951 WB Image

KO/KD

Wild-type (WT) and Cyclophilin F knockout (KO) 293T cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Cyclophilin F antibody (GTX117951) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cyclophilin F antibody [N1C3] used in Western Blot (WB). GTX117951

GTX117951 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Cyclophilin F antibody (GTX117951) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cyclophilin F antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX117951

GTX117951 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using PPIF(GTX117951) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Cyclophilin F antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117951

GTX117951 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using PPIF(GTX117951) antibody at 1:500 dilution.

Anti-Cyclophilin F antibody [N1C3] used in Western Blot (WB). GTX117951

GTX117951 WB Image

Mouse tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Cyclophilin F antibody (GTX117951) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cyclophilin F antibody [N1C3] used in Western Blot (WB). GTX117951

GTX117951 WB Image

Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Cyclophilin F antibody (GTX117951) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Ogawa T et al. Physiol Rep 2023; 11 (4) : e15608 Downregulation of extramitochondrial BCKDH and its uncoupling from AMP deaminase in type 2 diabetic OLETF rat hearts.
Application : WB
Reactivity : Rat
Danielle E Anderson et al. Proc Natl Acad Sci U S A 2021; 118 (39) Orthogonal genome-wide screens of bat cells identify MTHFD1 as a target of broad antiviral therapy.
Danielle E Anderson et al. bioRxiv 2020; (Epub) Orthogonal genome-wide screenings in bat cells identify MTHFD1 as a target of broad antiviral therapy.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

There are currently no reviews for Cyclophilin F antibody [N1C3] (GTX117951). Be the first to share your experience with this product.
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169