APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500~1:1000 |
1:50~1:100 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 150mM NaCl, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
The antiserum was produced against synthesized peptide derived from human EHHADH (476-525).
Purification
Purified by antigen-affinity chromatography
From serum
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
ECHD , EHHADH , FRTS3 , LBFP , LBP , LPBE , PBFE , enoylCoA hydratase and 3hydroxyacyl CoA dehydrogenase , enoyl-CoA, hydratase/3-hydroxyacyl CoA dehydrogenase
Cellular Localization
Peroxisome,Cytoplasm,Cytoskeleton
Background
The protein encoded by this gene is a bifunctional enzyme and is one of the four enzymes of the peroxisomal beta-oxidation pathway. The N-terminal region of the encoded protein contains enoyl-CoA hydratase activity while the C-terminal region contains 3-hydroxyacyl-CoA dehydrogenase activity. Defects in this gene are a cause of peroxisomal disorders such as Zellweger syndrome. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]
Database
Research Area
DATA IMAGES
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GTX87989 WB Image
WB analysis of A549 cell lysates using GTX87989 EHHADH antibody. The lane on the right is blocked with the synthesized peptide.
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GTX87989 IHC-P Image
IHC-P analysis of human breast carcinoma tissue using GTX87989 EHHADH antibody. The picture on the right is blocked with the synthesized peptide.
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REFERENCE
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REVIEW
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