GeneTex
United States (US)

EasyBlot anti Rabbit IgG (HRP)

GTX221666-01_IP_1_18121410_551.jpg
GTX221666-01_IP_2_18121410_450.jpg
GTX221666-01_42030_20150430_WB_Ag_18121410_148.jpg
easy blot_new_1_18121410_697.jpg

Cat No. GTX221666-01

Application WB
Reactivity Rabbit
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
Not tested in other applications.

Positive Control

native-rabbit IgG
PROPERTIES

Form

Liquid

Buffer

0.05M Tris, 0.15M NaCl (pH7.4), 1%BSA, 40% Glycerol (pH7). 0.005% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.318mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Rabbit

Target Isotype

IgG

Purification

Affinity purified by Protein G.

Conjugation

Horseradish peroxidase(HRP)

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
DATA IMAGES
GTX221666-01_IP_1_18121410_551.jpg

GTX221666-01 WB Image

Comparison of western blot analysis after immunoprecipitation with traditional secondary antibody, EasyBlot anti-Rabbit IgG (HRP) (GTX221666-01) and EasyBlot anti-Rabbit IgG kit (GTX225856-01). A,C,and E: E. coli lysate with overexpressed GST-tagged protein B,D,and F: IP result IP and 1st antibody: anti-GST antibody

GTX221666-01_IP_2_18121410_450.jpg

GTX221666-01 WB Image

Comparison of western blot analysis after immunoprecipitation with traditional secondary antibody (1) and EasyBlot anti-Rabbit IgG kit (GTX225856-01)(2). (A) Input, 20ug of HCT116 lysate. (B) and (C): HCT116 lysates were immunoprecipitated using 2.5 ug of normal rabbit IgG (B) or anti-p53 antibody (GTX102965)(C).  The precipitated protein was detected by GTX102965 diluted at 1:10000.

GTX221666-01_42030_20150430_WB_Ag_18121410_148.jpg

GTX221666-01 WB Image

EasyBlot anti Rabbit IgG (HRP) antibody detects native-rabbit IgG by western blot analysis.Recombinant Protein (8 and 0.1 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with EasyBlot anti Rabbit IgG (HRP) antibody (GTX221666-01) at a dilution of 1:5000.

easy blot_new_1_18121410_697.jpg

GTX221666-01 WB Image

GST-tagged protein (Mw: 45 kDa) was immunoprecipitated from E. coli lysate using 8 ug of anti-GST antibody (GTX110736). The precipitated protein was detected by GTX110736 diluted at 1:3000.

A: EasyBlot
B: Goat anti-rabbit antibody

REFERENCE
REVIEW

EasyBlot anti Rabbit IgG (HRP) Cat No. GTX221666-01

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 02-Feb-2018
Score :
Application Tested : WB
Sample : HSC2 cells
Lane Description : Immunoprecipitation of Tel2 protein. Input, HSC2 cells (Lane 1). HSC2 cells subjected to immunoprecipitation using Tel2 antibody (Lane 2) or normal rabbit IgG (Lane 3). The precipitated protein was detected by RUVBL1 antibody. EasyBlot anti-Rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent in Western blot and can avoid the interference of IgG heavy chains and light chains.
Application Reference
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 199
$ 89