PRODUCT
Summary
EpCAM antibody recognizes epithelial cell adhesion molecule (EpCAM), a transmembrane glycoprotein with a predicted molecular weight of ~35 kDa. EpCAM is upregulated in epithelial cells during epithelial–mesenchymal transition (EMT) and is used as a diagnostic marker of many carcinomas and circulating tumor cells. Detection of EpCAM by immunohistochemistry is important for the diagnosis of certain epithelial malignancies.
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
1:100-1:1000 |
Assay dependent |
Assay dependent |
Not tested in other applications.
Calculated MW
Negative Control
HeLa
Product Note
This antibody was raised against human EpCAM Extracellular domain.
We do not recommend use of this product for Mouse samples.
Predict Reactivity
Mouse, Rat, Sheep, Bovine, Pig, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the Extracellular domain of human EpCAM. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
RRID
AB_2909924
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
epithelial cell adhesion molecule , DIAR5 , EGP-2 , EGP314 , EGP40 , ESA , HNPCC8 , KS1/4 , KSA , M4S1 , MIC18 , MK-1 , TACSTD1 , TROP1
Background
This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq, Dec 2008]
Database
Research Area
DATA IMAGES
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GTX635967 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with EpCAM antibody [GT25512] (GTX635967) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX2131
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GTX635967 IHC-P Image
EpCAM antibody [GT25512] detects EpCAM protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded MDA-MB-231 xenograft.EpCAM stained by EpCAM antibody [GT25512] (GTX635967) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX635967 ICC/IF Image
EpCAM antibody [GT25512] detects EpCAM protein at cell membrane by immunofluorescent analysis.Sample: HCT116 and HeLa cells were fixed in ice-cold MeOH for 5 min.Green: EpCAM stained by EpCAM antibody [GT25512] (GTX635967) diluted at 1:500.
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GTX635967 FACS Image
EpCAM antibody[GT25512] (GTX635967) detects EpCAM by flow cytometry analysis.
Sample: HeLa and HCT-116
Blue: Mouse IgG isotype control.
Red: EpCAM antibody[GT25512] (GTX635967) dilution: 1:50.
Acquisition of 20,000 events were collected for FACS analysis.
HCT116 (EpCAM positive), HeLa (EpCAM negative)
HCT116 (EpCAM positive), HeLa (EpCAM negative)
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REFERENCE
There are currently no references for EpCAM antibody [GT25512] (GTX635967). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for EpCAM antibody [GT25512] (GTX635967). Be the first to share your experience with this product.