GeneTex
United States (US)

EpCAM antibody [rVU-1D9]

Anti-EpCAM antibody [rVU-1D9] used in IHC (Paraffin sections) (IHC-P). GTX34701

Cat No. GTX34701

Host Mouse
Clonality Monoclonal
Clone Name rVU-1D9
Isotype IgG1
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5-1ug/ml
ICC/IF 1-2ug/ml
IHC-P 0.5-1ug/ml for 30 minutes at RT

Note :

IHC-P
Boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes

Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Specificity/Sensitivity

This antibody reacts with the first EGF repeat in the extracellular domain of Ep-CAM. It is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas with the exception of adult squamous epithelium, hepatocytes and gastric epithelial cells. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.
PROPERTIES

Form

Liquid

Buffer

Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Immunogen

Small cell lung carcinoma cells

Purification

recombinant MAb purified Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Epithelial Cell Adhesion Molecule,Diar5,Egp-2,Egp314,Egp40,Esa,Hnpcc8,Ks1/4,Ksa,M4S1,Mic18,Mk-1,Tacstd1,Trop1,Epcam

Background

This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq, Dec 2008]

Database

Research Area

DATA IMAGES
Anti-EpCAM antibody [rVU-1D9] used in IHC (Paraffin sections) (IHC-P). GTX34701

GTX34701 IHC-P Image

Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Ep-CAM Recombinant Mouse Monoclonal Antibody (rVU-1D9).

Package List Price ($)
$ 329