APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1-3μg/ml |
3.75μg/ml |
Note :
IHC-P
Human Small Intestine shows strong staining of enterocytes.
|
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89327-PEP).
Predict Reactivity
Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-QDYEEKTKKAER, from the internal region of the protein sequence according to NP_003370.2.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
ezrin , CVIL , CVL , HEL-S-105 , VIL2
Cellular Localization
Apical cell membrane , Cytoplasm, cytoskeleton
Background
The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for this gene. Alternatively spliced variants have also been described for this gene. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
|
GTX89327 WB Image
WB analysis of human placenta lysate using GTX89327 Ezrin antibody, Internal. Dilution : 1μg/ml Loading : 35μg protein in RIPA buffer
|
|
GTX89327 IHC-P Image
IHC-P analysis of human small intestine using GTX89327 Ezrin antibody, Internal. Antigen retrieval : citrate buffer pH 6 Dilution : 3.8μg/ml
|
|
GTX89327 IHC-P Image
IHC-P analysis of human kidney using GTX89327 Ezrin antibody, Internal. Antigen retrieval : citrate buffer pH 6 Dilution : 3.75μg/ml
|
REFERENCE
There are currently no references for Ezrin antibody, Internal (GTX89327). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Ezrin antibody, Internal (GTX89327). Be the first to share your experience with this product.