GeneTex
United States (US)

FGF2 recombinant protein

Cat No. GTX65429

Application ELISA, Functional Assay, Apuri, Blocking
Reactivity Rat
Species Rat
APPLICATION

Application Note

The ED50, calculated by the dose-dependant proliferation of BALB/3T3 cells is <0.2 ng/ml, corresponding to a specific activity of 5x10^6 IU/mg.

Calculated MW

16.3 kDa. ( Note )
PROPERTIES

Form

Lyophilized powder

Buffer

1 mg/ml solution after extensive dialysis against 20 mM phosphate buffer, pH 7.4 and 130 mM NaCl.

Storage

Reconstitute in sterile water to 0.1–1 mg /ml. The solution can then further diluted to other aqueous solutions. Stored at -20ºC for future use.

Antigen Species

Rat

Expression System

E. coli

Purification


Purity was assessed by SDS-PAGE (≥97%) and by HPLC (≥97%).

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

FGFb, HBGH-2, Prostatropin, HBGF2, HBGF-2, FGF2, FGF-2, HBGH2, Fibroblast Growth Factor, FGF-b, FGF 2, FGF b, HBGH 2, HBGF 2

Background

FGF-2 is a basic heparin binding growth factor that stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal and endothelial cells. The recombinant rat FGF-2 is a single, non-glycosylated chain containing 145 amino acids and having a molecular weight of 16.3 kDa.

Research Area