APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
1:100-1:1000 |
1:100-1:500 |
Not tested in other applications.
Calculated MW
Positive Control
HeLa
Predict Reactivity
Bovine, Chicken(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.92 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human Filamin B. The exact sequence is proprietary.
Purification
Affinity purified by Protein G.
Conjugation
Unconjugated
RRID
AB_2888309
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
filamin B , ABP-278 , ABP-280 , AOI , FH1 , FLN-B , FLN1L , LRS1 , SCT , TABP , TAP
Cellular Localization
Isoform 1: Cytoplasm , cell cortex , Cytoplasm , cytoskeleton , myofibril , sarcomere , Z-disk , Isoform 2: Cytoplasm , Isoform 3: Cytoplasm , Isoform 6: Cytoplasm
Background
This gene encodes a member of the filamin family. The encoded protein interacts with glycoprotein Ib alpha as part of the process to repair vascular injuries. The platelet glycoprotein Ib complex includes glycoprotein Ib alpha, and it binds the actin cytoskeleton. Mutations in this gene have been found in several conditions: atelosteogenesis type 1 and type 3; boomerang dysplasia; autosomal dominant Larsen syndrome; and spondylocarpotarsal synostosis syndrome. Multiple alternatively spliced transcript variants that encode different protein isoforms have been described for this gene. [provided by RefSeq, Nov 2009]
Database
Research Area
DATA IMAGES
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GTX632360 WB Image
Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Filamin B antibody [GT1372] (GTX632360) diluted at 1:500.
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GTX632360 IP Image
Immunoprecipitation of Filamin B protein from HeLa whole cell extracts using 5 μg of Filamin B antibody (GTX632360). Western blot analysis was performed using Filamin B antibody (GTX632360) diluted at 1:500. EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
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GTX632360 IHC-P Image
Filamin B antibody [GT1372] detects Filamin B protein at cytoplasm in mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. Filamin B antibody [GT1372] (GTX632360) diluted at 1:250.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX632360 IHC-P Image
Filamin B antibody [GT1372] detects Filamin B protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Filamin B antibody [GT1372] (GTX632360) diluted at 1:250.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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REFERENCE
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REVIEW
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