APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:10000 |
1:100-1:1000 |
1:100-1:1000 |
1:100-1:1000 |
1:100-1:500 |
Not tested in other applications.
Calculated MW
Positive Control
NIH-3T3 , JC
Predict Reactivity
Chicken, Pig, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.62 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human Gephyrin. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_11164321
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
gephyrin , GEPH , GPH , GPHRYN , HKPX1 , MOCODC
Cellular Localization
Cell junction , synapse , synapse , postsynaptic cell membrane; Peripheral membrane protein; Cytoplasmic side , Cytoplasm , cytoskeleton
Background
This gene encodes a neuronal assembly protein that anchors inhibitory neurotransmitter receptors to the postsynaptic cytoskeleton via high affinity binding to a receptor subunit domain and tubulin dimers. In nonneuronal tissues, the encoded protein is also required for molybdenum cofactor biosynthesis. Mutations in this gene may be associated with the neurological condition hyperplexia and also lead to molybdenum cofactor deficiency. Numerous alternatively spliced transcript variants encoding different isoforms have been described; however, the full-length nature of all transcript variants is not currently known. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX109734 IHC-Fr Image
Gephyrin antibody [N2C1], Internal detects Gephyrin protein expression by immunohistochemical analysis. Sample: Frozen sectioned E13.5 Rat brain. Green: Gephyrin protein stained by Gephyrin antibody [N2C1], Internal (GTX109734) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
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GTX109734 IHC-P Image
Gephyrin antibody [N2C1], Internal detects Gephyrin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.Gephyrin stained by Gephyrin antibody [N2C1], Internal (GTX109734) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX109734 WB Image
Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC 7.5% SDS PAGE GTX109734 diluted at 1:1000
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GTX109734 IP Image
Immunoprecipitation of Gephyrin protein from A431 whole cell extracts using 5 μg of Gephyrin antibody [N2C1], Internal (GTX109734). Western blot analysis was performed using Gephyrin antibody [N2C1], Internal (GTX109734). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
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GTX109734 IHC-P Image
Immunohistochemical analysis of paraffin-embedded mouse brain, using Gephyrin(GTX109734) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX109734 ICC/IF Image
Gephyrin antibody [N2C1], Internal detects Gephyrin protein by immunofluorescent analysis. Sample: DIV10 rat E18 primary hippocampal neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: Gephyrin protein stained by Gephyrin antibody [N2C1], Internal (GTX109734) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
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GTX109734 WB Image
Human tissue extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Gephyrin antibody [N2C1], Internal (GTX109734) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX109734 WB Image
Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Gephyrin antibody [N2C1], Internal (GTX109734) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX109734 WB Image
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Gephyrin antibody [N2C1], Internal (GTX109734) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
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