GeneTex
United States (US)

HIV1 Reverse Transcriptase antibody [AS35] (Biotin)

Cat No. GTX16162

Host Mouse
Clonality Monoclonal
Clone Name AS35
Isotype IgG1
Application IP, ELISA
Reactivity Human Immunodeficiency virus
APPLICATION

Application Note

ELISA: This antibody has been used as solution phase ab at 2 μg/ml in a two site ELISA with AS34. Typically the ELISA has a working range of 2-500 ng/ml and a sensitivity of <1 ng/ml HIV-RT. IP: This antibody has been used to immunoprecipitate > 90% HIV-RT in solution using a double-antibody method. Immunoprecipitation has also been accomplished using Protein G agarose. Optimal dilutions/concentrations should be determined by the end user.
PROPERTIES

Form

Liquid

Buffer

Preservative: 0.08% Sodium Azide; Constituents: Ca2+ & Mg2+ free Dulbecco's PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human Immunodeficiency virus

Immunogen

Recombinant HIV1 Reverse Transcriptase

Purification

Protein G purified
Antibody Purity > 95 % by SDS PAGE

Conjugation

Biotin

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

HIV RT Immuno Deficiency Virus Reverse Transcriptase

Research Area

Package List Price ($)
$ 329