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IL7 Receptor alpha (phospho Tyr449) antibody

Orthogonal
Anti-IL7 Receptor alpha (phospho Tyr449) antibody used in IHC (Paraffin sections) (IHC-P). GTX85604
Anti-IL7 Receptor alpha (phospho Tyr449) antibody used in Western Blot (WB). GTX85604

Cat. No. GTX85604

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
25 μg ($399)
See all IL7 Receptor alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000 - 1:10000
IHC-P 5 μg/ml
ELISA 1:100000

Note :

IHC-P
Heat induced antigen retrieval in pH 6.0 citrate buffer
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Synthetic peptide corresponding to residues near Y449 of mouse IL-7 protein

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Cellular Localization

Membrane

Background

Interleukin-7 is a glycoptorein involved in the regulation of lymphopoiesis. Response of cells to IL7 is dependent on the presence of the interleukin 7 receptor (IL7R); the active receptor is a alpha/gamma chain heterodimer. The gamma(c) chain, which also associates with the interleukin-2 receptor, serves primarily to activate signal transduction by the IL7R complex, while the alpha chain of IL7R determines specific signaling events through its association with cytoplasmic signaling molecules. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-IL7 Receptor alpha (phospho Tyr449) antibody used in IHC (Paraffin sections) (IHC-P). GTX85604

GTX85604 IHC-P Image

IHC-P analysis of human colon, malt tissue using GTX85604 IL7 Receptor alpha (phospho Tyr449) antibody.
Antigen retrieval : Heat-induced antigen retrieval
Dilution : 5 μg/ml

Anti-IL7 Receptor alpha (phospho Tyr449) antibody used in Western Blot (WB). GTX85604

GTX85604 WB Image

Orthogonal

WB analysis of thymocyte D1 cells treated with or without IL7 (50 ng/ml) using GTX85604 IL7 Receptor alpha (phospho Tyr449) antibody.
The cell lysate was immunoprecipitated with anti-Phosphotyrosine antibody and then immunobloted by IL7 Receptor alpha (phospho Tyr449) antibody
Dilution : 1:10000

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399