GeneTex
United States (US)

LC3A antibody

Anti-LC3A antibody used in IHC (Paraffin sections) (IHC-P). GTX48634
Anti-LC3A antibody used in Western Blot (WB). GTX48634
Anti-LC3A antibody used in IHC (Paraffin sections) (IHC-P). GTX48634
Anti-LC3A antibody used in Western Blot (WB). GTX48634
Anti-LC3A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX48634
Anti-LC3A antibody used in Western Blot (WB). GTX48634

Cat No. GTX48634

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr
Reactivity Human, Mouse, Rat, Zebrafish, Bovine
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:2500
ICC/IF 1:100
IHC-P 1:100 - 1:400
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

14 kDa. ( Note )

Specificity/Sensitivity

Although specificity between LC3A and LC3B has not been tested, this antibody was created to a peptide that has 100% identity to LC3A and 62% identity to LC3B.
PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA,, 50% Glycerol, 0.05% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.20 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Genomic sequence made to an N-terminal portion of the human LC3A protein [Swiss-Prot# Q9H492].

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Microtubule Associated Protein 1 Light Chain 3 Alpha,Atg8E,Lc3,Lc3A,Map1Alc3,Map1Blc3,Map1Lc3A

Cellular Localization

Cytoplasm,Endomembrane system, Lipid-anchor,Cytoplasmic vesicle,autophagosome membrane, Lipid-anchor

Background

MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-LC3A antibody used in IHC (Paraffin sections) (IHC-P). GTX48634

GTX48634 IHC-P Image

IHC-P analysis of mouse liver tissue using GTX48634 LC3A antibody.
Dilution : 1:300

Anti-LC3A antibody used in Western Blot (WB). GTX48634

GTX48634 WB Image

WB analysis of human brain tissue lysate using GTX48634 LC3A antibody.

Anti-LC3A antibody used in IHC (Paraffin sections) (IHC-P). GTX48634

GTX48634 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX48634 LC3A antibody.
Dilution : 1:300

Anti-LC3A antibody used in Western Blot (WB). GTX48634

GTX48634 WB Image

WB analysis of Neuro2a cell lysate using GTX48634 LC3A antibody.

Anti-LC3A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX48634

GTX48634 ICC/IF Image

ICC/IF analysis of HeLa cells treated with 50uM chloroquine using GTX48634 LC3A antibody.
Green : primary antibody
Red : Tubulin
Blue : DAPI

Anti-LC3A antibody used in Western Blot (WB). GTX48634

GTX48634 WB Image

WB analysis of HeLa and Neuro2a cells treated with or without 50 uM chloroquine(CQ) for 24 hours using GTX48634 LC3A antibody.
Dilution : 2 μg/ml

Package List Price ($)
$ 249