Reconstitute in water to a concentration of 0.1-1.0 mg/ml. The solution can then be diluted into other aqueous buffers and stored at 4ºC for 1 week or –20ºC for future use.
Purity was assessed by SDS-PAGE (≥95%) and by HPLC (≥95%).
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Leukemia Inhibitory Factor
Leukemia Inhibitory Factor (LIF) is a lymphoid factor that promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. LIF has several functions such as cholinergic neuron differentiation, control of stem cell pluripotency, bone & fat metabolism, mitogenesis of factor dependent cell lines & promotion of megakaryocyte production in vivo. Human and 22 LIF exhibit a 78% identity in its amino acid sequence. Human LIF is as active on human cells as is it is on 22 cells, though 22 LIF is about 1000 fold less active on human cells, than human LIF. Recombinant human LIF produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 180 amino acids and having a molecular mass of 19.7 kDa.