APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , HeLa , HepG2 , mouse brain , rat brain
Predict Reactivity
Bovine, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.67 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human MARS. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1950888
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
methionyl-tRNA synthetase , CMT2U , ILFS2 , ILLD , METRS , MRS , MTRNS , SPG70
Cellular Localization
Cytoplasm
Background
Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene belongs to the class I family of tRNA synthetases. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX109864 WB Image
Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MARS antibody [N3C2], Internal (GTX109864) diluted at 1:1000.
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GTX109864 ICC/IF Image
MARS antibody [N3C2], Internal detects MARS protein at cytoplasm by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MARS protein stained by MARS antibody [N3C2], Internal (GTX109864) diluted at 1:500. Blue: Hoechst 33342 staining.
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GTX109864 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MARS antibody [N3C2], Internal (GTX109864) diluted at 1:1000.
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REFERENCE
There are currently no references for MARS antibody [N3C2], Internal (GTX109864). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for MARS antibody [N3C2], Internal (GTX109864). Be the first to share your experience with this product.