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MED1 antibody

Anti-MED1 antibody used in Western Blot (WB). GTX55164
Anti-MED1 antibody used in IHC (Paraffin sections) (IHC-P). GTX55164
Anti-MED1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55164

Cat. No. GTX55164

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399)
See all MED1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
ICC/IF 1:100 - 1:500
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

168 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.42% Potassium Phosphate, 0.87% NaCl, 30% Glycerol

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of MED1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mediator complex subunit 1 , CRSP1 , CRSP200 , DRIP205 , DRIP230 , PBP , PPARBP , PPARGBP , RB18A , TRAP220 , TRIP2

Cellular Localization

Nucleus

Background

The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. It also regulates p53-dependent apoptosis and it is essential for adipogenesis. This protein is known to have the ability to self-oligomerize. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-MED1 antibody used in Western Blot (WB). GTX55164

GTX55164 WB Image

WB analysis of HeLa (A), HUVEC (B), Jurkat (C) whole cell lysates using GTX55164 MED1 antibody.

Anti-MED1 antibody used in IHC (Paraffin sections) (IHC-P). GTX55164

GTX55164 IHC-P Image

IHC-P analysis of formalin fixed human breast cancer tissue section using GTX55164 MED1 antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

Anti-MED1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55164

GTX55164 ICC/IF Image

ICC/IF analysis of formalin-fixed HUVEC cells using GTX55164 MED1 antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399