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Mucin 5AC antibody [45M1]

Anti-Mucin 5AC antibody [45M1] used in IHC (Paraffin sections) (IHC-P). GTX34872
Anti-Mucin 5AC antibody [45M1] used in IHC (Paraffin sections) (IHC-P). GTX34872
Anti-Mucin 5AC antibody [45M1] used in IHC (Paraffin sections) (IHC-P). GTX34872

Cat No. GTX34872

Host Mouse
Clonality Monoclonal
Clone Name 45M1
Isotype IgG1
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat, Rabbit, Cat, Chicken, Pig, Monkey, Hedgehog
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF 1-2ug/ml
IHC-P 1-2ug/ml for 30 minutes at RT

Note :

IHC-P
Boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes

Not tested in other applications.

Specificity/Sensitivity

This MAb recognizes the peptide core of gastric mucin M1 (recently identified as Mucin 5AC).ĀIts epitope is located in the C-terminal cysteine rich part of the peptide core of MUC5AC. Its epitope is destroyed by beta-mercaptoethanol but not by periodate treatment. This mucin is present in primary ovarian mucinous cancer but usually absent in colorectal adenocarcinoma, thus showing an expression pattern opposite to MUC2. Together with a panel of antibodies, Anti-MUC5AC may be useful for differential identification of primary mucinous ovarian tumors from colon adenocarcinoma metastatic to the ovary. MUC5AC antibodies may also be useful for identification of intestinal metaplasia as well as in the identification of pancreatic carcinoma and pre-cancerous changes vs. normal pancreas.
PROPERTIES

Form

Liquid

Buffer

Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Immunogen

M1 mucin preparation from the fluid of an ovarian mucinous cyst belonging to an O Le(a-b) patient

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Mucin 5Ac, Oligomeric Mucus/Gel-Forming,Muc5,Tbm,Leb,Mucin,Muc5Ac
DATA IMAGES
Anti-Mucin 5AC antibody [45M1] used in IHC (Paraffin sections) (IHC-P). GTX34872

GTX34872 IHC-P Image

Formalin-fixed, paraffin-embedded human Stomach stained with MUC5AC Monoclonal Antibody (45M1).

Anti-Mucin 5AC antibody [45M1] used in IHC (Paraffin sections) (IHC-P). GTX34872

GTX34872 IHC-P Image

Formalin-fixed, paraffin-embedded Rat Stomach stained with MUC5AC Monoclonal Antibody (45M1).

Anti-Mucin 5AC antibody [45M1] used in IHC (Paraffin sections) (IHC-P). GTX34872

GTX34872 IHC-P Image

Formalin-fixed, paraffin-embedded human Gastric Carcinoma stained with MUC5AC Monoclonal Antibody (45M1).

REFERENCE
Package List Price ($)
$ 329