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Myosin Light Chain 2 (MLC-2v) antibody

Anti-Myosin Light Chain 2 (MLC-2v) antibody used in Western Blot (WB). GTX33339
Anti-Myosin Light Chain 2 (MLC-2v) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33339

Cat No. GTX33339

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:100
Not tested in other applications.

Calculated MW

19 kDa. ( Note )

Specificity/Sensitivity

This antibody detects phosphorylated Ser19 Myosin Light Chain 2. The sequence around Ser19 is conserved among MYL9, MYL12A and MYL12B protein.
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-166 of human MYL2 (NP_000423.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Myosin Light Chain 2,Cmh10,Mlc-2S/V,Mlc2,Myl2

Background

Thus gene encodes the regulatory light chain associated with cardiac myosin beta (or slow) heavy chain. Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in this gene are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Anti-Myosin Light Chain 2 (MLC-2v) antibody used in Western Blot (WB). GTX33339

GTX33339 WB Image

WB analysis of various samples using GTX33339 Myosin Light Chain 2 (MLC-2v) antibody.
Dilution : 1:3000
Loading : 25μg per lane

Anti-Myosin Light Chain 2 (MLC-2v) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33339

GTX33339 ICC/IF Image

ICC/IF analysis of HeLa cell using GTX33339 Myosin Light Chain 2 (MLC-2v) antibody.
Blue : DAPI

Package List Price ($)
$ 329