GeneTex
United States (US)

PNK antibody [N1C3]

GTX108569_40030_WB_19011507_309.jpg
GTX108569_IHC_19011507_217.jpg

Cat No. GTX108569

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Positive Control

A549 , HeLa , HepG2

Predict Reactivity

Mouse, Rat(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PNK. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Polynucleotide Kinase 3'-Phosphatase,Aoa4,Eiee10,Mcsz,Pnk,Pnkp

Background

This locus represents a gene involved in DNA repair. In response to ionizing radiation or oxidative damage, the protein encoded by this locus catalyzes 5' phosphorylation and 3' dephosphorylation of nucleic acids. Mutations at this locus have been associated with microcephaly, seizures, and developmental delay.

Database

DATA IMAGES
GTX108569_40030_WB_19011507_309.jpg

GTX108569 WB Image

Sample (30 ug of whole cell lysate)
A: A549
7.5% SDS PAGE
GTX108569 diluted at 1:2000

GTX108569_IHC_19011507_217.jpg

GTX108569 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 xenograft , using PNK(GTX108569) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169