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PNK antibody

Anti-PNK antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64651
Anti-PNK antibody used in IHC (Paraffin sections) (IHC-P). GTX64651
Anti-PNK antibody used in IHC (Paraffin sections) (IHC-P). GTX64651
Anti-PNK antibody used in Western Blot (WB). GTX64651
Anti-PNK antibody used in IHC (Paraffin sections) (IHC-P). GTX64651

Cat. No. GTX64651

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all PNK products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:100
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 60-320 of human PNKP (NP_009185.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

polynucleotide kinase 3'-phosphatase , AOA4 , EIEE10 , MCSZ , PNK

Cellular Localization

Nucleus

Background

This locus represents a gene involved in DNA repair. In response to ionizing radiation or oxidative damage, the protein encoded by this locus catalyzes 5' phosphorylation and 3' dephosphorylation of nucleic acids. Mutations at this locus have been associated with microcephaly, seizures, and developmental delay.[provided by RefSeq, Sep 2010]

Database

Research Area

DATA IMAGES

Anti-PNK antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64651

GTX64651 ICC/IF Image

ICC/IF analysis of GFP-RNF168 transgenic U2OS cells using GTX64651 PNK antibody.
Green : GFP-RNF168 fusion protein expression for DNA damage marker
Blue : DAPI

Anti-PNK antibody used in IHC (Paraffin sections) (IHC-P). GTX64651

GTX64651 IHC-P Image

IHC-P analysis of rat kidney tissue using GTX64651 PNK antibody.
Dilution : 1:100

Anti-PNK antibody used in IHC (Paraffin sections) (IHC-P). GTX64651

GTX64651 IHC-P Image

IHC-P analysis of human lung cancer tissue using GTX64651 PNK antibody.
Dilution : 1:100

Anti-PNK antibody used in Western Blot (WB). GTX64651

GTX64651 WB Image

WB analysis of various sample lysates using GTX64651 PNK antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-PNK antibody used in IHC (Paraffin sections) (IHC-P). GTX64651

GTX64651 IHC-P Image

IHC-P analysis of mouse heart tissue using GTX64651 PNK antibody.
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399