APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500 - 1:2000 |
| 1:50 - 1:200 |
| 1:50 - 1:100 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
A synthetic peptide of human PRMT5
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
protein arginine methyltransferase 5 , HRMT1L5 , HSL7 , IBP72 , JBP1 , SKB1 , SKB1Hs
Cellular Localization
Cytoplasm,Nucleus,Golgi apparatus
Background
This gene encodes an enzyme that belongs to the methyltransferase family. The encoded protein catalyzes the transfer of methyl groups to the amino acid arginine, in target proteins that include histones, transcriptional elongation factors and the tumor suppressor p53. This gene plays a role in several cellular processes, including transcriptional regulation, and the assembly of small nuclear ribonucleoproteins. A pseudogene of this gene has been defined on chromosome 4. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015]
Database
Research Area
DATA IMAGES
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GTX32810 IHC-P Image
IHC-P analysis of human colon tissue using GTX32810 PRMT5 antibody.
Dilution : 1:100
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GTX32810 IHC-P Image
IHC-P analysis of mouse brain tissue using GTX32810 PRMT5 antibody.
Dilution : 1:100
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GTX32810 ICC/IF Image
ICC/IF analysis of H9C2 cells using GTX32810 PRMT5 antibody.
Blue : DAPI
Dilution : 1:100
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GTX32810 ICC/IF Image
ICC/IF analysis of U2OS cells using GTX32810 PRMT5 antibody.
Blue : DAPI
Dilution : 1:100
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GTX32810 ICC/IF Image
ICC/IF analysis of L929 cells using GTX32810 PRMT5 antibody.
Blue : DAPI
Dilution : 1:100
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GTX32810 IHC-P Image
IHC-P analysis of rat brain tissue using GTX32810 PRMT5 antibody.
Dilution : 1:100
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GTX32810 WB Image
WB analysis of various sample lysates using GTX32810 PRMT5 antibody.
Dilution : 1:1000
Loading : 25μg per lane
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REFERENCE
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REVIEW
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