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RAI3 antibody [N1], N-term

Anti-RAI3 antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX108135
Anti-RAI3 antibody [N1], N-term used in Western Blot (WB). GTX108135
Anti-RAI3 antibody [N1], N-term used in Western Blot (WB). GTX108135
Anti-RAI3 antibody [N1], N-term used in Western Blot (WB). GTX108135

Cat. No. GTX108135

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all RAI3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Positive Control

HeLa , HepG2 , A549 , H1299 , HCT116

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide encompassing a sequence within the Extracellular domain of human RAI3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950445

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

G protein-coupled receptor class C group 5 member A , GPCR5A , PEIG-1 , RAI3 , RAIG1 , TIG1

Cellular Localization

Cell membrane; Multi-pass membrane protein , Cytoplasmic vesicle membrane; Multi-pass membrane protein

Background

This gene encodes a member of the type 3 G protein-coupling receptor family, characterized by the signature 7-transmembrane domain motif. The encoded protein may be involved in interaction between retinoid acid and G protein signalling pathways. Retinoic acid plays a critical role in development, cellular growth, and differentiation. This gene may play a role in embryonic development and epithelial cell differentiation. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-RAI3 antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX108135

GTX108135 IHC-P Image

Immunohistochemical analysis of paraffin-embedded CAL27 xenograft, using RAI3(GTX108135) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RAI3 antibody [N1], N-term used in Western Blot (WB). GTX108135

GTX108135 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with RAI3 antibody [N1], N-term (GTX108135) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RAI3 antibody [N1], N-term used in Western Blot (WB). GTX108135

GTX108135 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with RAI3 antibody [N1], N-term (GTX108135) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RAI3 antibody [N1], N-term used in Western Blot (WB). GTX108135

GTX108135 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with RAI3 antibody [N1], N-term (GTX108135) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Chang YT et al. Am J Cancer Res 2020; 10 (1) : 12-37 Pancreatic cancer-derived small extracellular vesical Ezrin regulates macrophage polarization and promotes metastasis.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169