For WB: Use at a dilution of 1:1,000. A ~48 kDa band can be seen in a Western blot analysis, using rat brain hippocampal homogenate. Tested in TBST (20 mM Tris/HCl (7.6), 137 mM NaCl, 1% Tween-20) containing 5% nonfat milk and 0.05% NaN3. Optimal dilutions/concentrations should be determined by the researcher.
This antibody is specific for Retinoic acid receptor, alpha isotype.
10 mM Hepes, pH7.5, 150 mM NaCl, 100 ug/ml BSA and 50% glycerol
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
0.5mg/ml(Please refer to the vial label for the specific concentration.)
Protein G purified
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
NR1B1, 5914, RAR alpha, RARA, P10276, 180240, RAR
Retinoids can reverse various premalignant lesions as well as prevent some second primary tumors. The effects of retinoids are regulated by retinoic acid receptors (RAR) and retinoid X receptors, which act as ligand-activated transcription factors. Ligand-dependent transcriptional activation of RARs is a multistep process that ends with the formation of a multimeric co-activator complex on regulated promoters (1,2).