GeneTex
United States (US)

SAPK4 (Active) recombinant protein

Cat No. GTX65221

Application ELISA, Functional Assay, Apuri, Blocking
Reactivity Human
Species Human
APPLICATION

Application Note

259 nmol phosphate incorporated into MBP protein per minute per mg protein at 30ºC for 15 minutes using a final concentration of 50 uM ATP (0.83 uCi/assay).

Calculated MW

71.0 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol

Storage

Store at -80ºC. Product is stable for at least 6-12 months.

Concentration

0.1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Expression System

Baculovirus (Sf9 insect cells)

Purification


Purity was assessed by SDS-PAGE (≥80%) and by HPLC.

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

p38, mitogenactivated protein kinase 13, MAPK13, mitogen-activated protein kinase 13, mitogenactivated protein kinase13

Background

Mitogen-activated protein kinase (MAPK) cascades represent one of the major signal systems used by eukaryotic cells to transduce extracellular signals into cellular responses, and involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The stress-activated protein kinase 4 (SAPK4), or p38 delta, is a member of the MAPK family that are activated by chemical and environmental stresses as well as by proinflammatory cytokines. SAPK4 has a TGY dual phosphorylation motif and is activated in response to cellular stresses and proinflammatory cytokines (1). MAP kinase kinases 3, and 6 can phosphorylate and activate this kinase. Transcription factor ATF2, and microtubule dynamics regulator stathmin have been shown to be the substrates of this kinase (2).

Research Area

Package List Price ($)
$ 349