APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500 - 1:1000 |
1:100 |
1:10 - 1:500 |
Not tested in other applications.
Calculated MW
Product Note
This is specific for Set7/9 (a histone H3 lysine 4 methyltransferase) and does not recognize PR-Set7 (a histone H4 lysine 20 methyltransferase).
PROPERTIES
Form
Liquid
Buffer
Serum
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Antigen Species
Human
Immunogen
Two synthethic peptides corresponding to amino acids 131-145 (GEVNEDGEMTGEKIA) and 336-352 (GYDHSPPGKSGPEAPEW) of human SETD7 were used as immunogen for this antibody.
Purification
Unpurified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
SET domain containing 7, histone lysine methyltransferase , KMT7 , SET7 , SET7/9 , SET9
Cellular Localization
Nucleus
Background
Histone methyltransferase that specifically monomethylates 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in the transcriptional activation of genes such as collagenase or insulin. Recruited by IPF1/PDX-1 to the insulin promoter, leading to activate transcription. Has also methyltransferase activity toward non-histone proteins such as p53/TP53, TAF10, and possibly TAF7 by recognizing and binding the [KR]-[STA]-K in substrate proteins. Monomethylates 'Lys-189' of TAF10, leading to increase the affinity of TAF10 for RNA polymerase II. Monomethylates 'Lys-372' of p53/TP53, stabilizing p53/TP53 and increasing p53/TP53-mediated transcriptional activation. Also able to demethylated 'Lys-372' of p53/TP53 in vitro.
Database
Research Area
DATA IMAGES
|
GTX13731 IHC-P Image
IHC-P analysis of human small intestine tissue using GTX13731 SETD7 antibody. Dilution : 1:100
|
|
GTX13731 WB Image
WB analysis of (A) MCF-7 and (B) HeLa cell lysate using GTX13731 SETD7 antibody. Dilution : 1:500
|
REFERENCE
There are currently no references for SETD7 antibody (GTX13731). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for SETD7 antibody (GTX13731). Be the first to share your experience with this product.