GeneTex
United States (US)

SIRP alpha antibody

Anti-SIRP alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31256
Anti-SIRP alpha antibody used in Western Blot (WB). GTX31256

Cat No. GTX31256

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, ELISA
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5 - 1 μg/mL
ICC/IF 1 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Positive Control

THP-1 Cell Slide(GTX25540) , THP-1 Cell Lysate(GTX27913)

Specificity/Sensitivity

Recognizes SIRP alpha 1, 2 and 3.
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

SIRP alpha antibody was raised against a peptide corresponding to 17 amino acids near the carboxy terminus of human SIRP alpha 1.The immunogen is located within the last 50 amino acids of SIRP alpha.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Signal Regulatory Protein Alpha,Bit,Cd172A,Mfr,Myd-1,P84,Ptpns1,Shps1,Sirp,Sirpa

Background

The protein encoded by this gene is a member of the signal-regulatory-protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. This protein can be phosphorylated by tyrosine kinases. The phospho-tyrosine residues of this PTP have been shown to recruit SH2 domain containing tyrosine phosphatases (PTP), and serve as substrates of PTPs. This protein was found to participate in signal transduction mediated by various growth factor receptors. CD47 has been demonstrated to be a ligand for this receptor protein. This gene and its product share very high similarity with several other members of the SIRP family. These related genes are located in close proximity to each other on chromosome 20p13. Multiple alternatively spliced transcript variants have been determined for this gene. [provided by RefSeq, Jul 20

Database

Research Area

DATA IMAGES
Anti-SIRP alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31256

GTX31256 ICC/IF Image

ICC/IF analysis of THP-1 cells using GTX31256 SIRP alpha antibody.
Working concentration : 1 μg/ml

Anti-SIRP alpha antibody used in Western Blot (WB). GTX31256

GTX31256 WB Image

WB analysis of THP-1 cell lysate using GTX31256 SIRP alpha antibody.
Working concentration : 0.5 μg/ml

Package List Price ($)
$ 329