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TIM-3 antibody [RMT3-23] (PE)

Anti-TIM-3 antibody [RMT3-23] (PE) used in Flow cytometry (FACS). GTX54056

Cat. No. GTX54056

Reference 1

Host

 Rat

Clonality

 Monoclonal

Clone Name

 RMT3-23

Isotype

 IgG3a

Application

 FACS

Reactivity

 Mouse
Package
100 μg ($329)
See all TIM-3 products
PRODUCT

Summary

The RMT3-23 antibody reacts with mouse CD366 which is also known as Tim-3 (T cell immunoglobulin and mucin domain containing protein-3). Tim-3 is expressed on activated monocytes, macrophages, dendritic cells, and mast cells. Additionally, Tim-3 is found on Th1 lymphocytes and functions as an inhibitory receptor involved in the maintenance of immune homeostasis and self tolerance.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
FACS Assay dependent
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM NaH₂PO₄, 150mM NaCl, 0.1% Gelatin

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Tim-3-Ig, consisting of amino acids 1-191 of mouse Tim-3, coupled with the Fc portion of mouse IgG2a.

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Phycoerythrin (PE)

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

hepatitis A virus cellular receptor 2 , TIM-3 , Tim3 , Timd3

Cellular Localization

Cell junction

Database

Research Area

DATA IMAGES

Anti-TIM-3 antibody [RMT3-23] (PE) used in Flow cytometry (FACS). GTX54056

GTX54056 FACS Image

FACS analysis of C57Bl/6 splenocytes stimulated for 3 days with ConA stained APC anti-mouse CD4 antibody, followed by staining with GTX54056 TIM-3 antibody [RMT3-23] (PE).
Right panel : Co-stained with APC anti-mouse CD4 antibody and GTX54056
Left panel : Co-stained with APC anti-mouse CD4 antibody and PE rat IgG2a isotype control
Antibody amount : 0.5 μg

REFERENCE

Clone Reference

Huang S et al. Mol Ther 2021; (Epub) Tim-3 regulates sepsis-induced immunosuppression by inhibiting the NF-κB signaling pathway in CD4 T cells.
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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 329