TNF alpha recombinant protein

Cat No. GTX65389

Application	ELISA, Functional Assay, Apuri, Blocking
Reactivity	Mouse
Species	Mouse

See all TNF alpha products

Datasheet

APPLICATION

Application Note

The ED50 as determined by the cytolysis of murine L929 cells in the presence of actinomycin D is < 0.1 ng/ml, corresponding to a specific activity of > 1x10^7 units/mg.

Calculated MW

17.5 kDa. ( Note )

PROPERTIES

Form

Lyophilized powder

Buffer

5 mM Tris, pH 8.0.

Storage

Reconstitute in water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4ºC for 1 week or –20ºC for future use.

Antigen Species

Mouse

Expression System

E. coli

Purification

Purity was assessed by SDS-PAGE (≥98%) and by HPLC (≥98%).

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Tumor necrosis factor- alpha, TNFA, TNF, Tumor necrosis factor alpha, Tumor necrosis factor alpha

Background

Tumor Necrosis Factor-alpha TNF-alpha ) is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells and certain other target cells. Murine TNF-alpha is a 17.5 kDa protein containing 156 amino acid residues.