GeneTex
United States (US)

VPS34 antibody [C1C3]

KO/KD
GTX107893_39988_20160818_WB_shRNA_watermark_19011507_216.jpg
GTX107893_WB_19011507_630.jpg

Cat No. GTX107893

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

102 kDa. ( Note )

Positive Control

A431 , 293T

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Dog, Pig, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.02mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human VPS34. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

MGC61518 antibody, VPS34 antibody, hVps34 antibody, PIK3C3 antibody, PI3K type 3 antibody, PI3-kinase type 3 antibody, phosphatidylinositol 3-kinase p100 subunit antibody, phosphatidylinositol 3-kinase catalytic subunit type 3 antibody, PtdIns-3-kinase type 3 antibody, "phosphoinositide-3-kinase, class 3 antibody"

Background

Catalytic subunit of the PI3K complex. Involved in the transport of lysosomal enzyme precursors to lysosomes.
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Database

Research Area

DATA IMAGES
GTX107893_39988_20160818_WB_shRNA_watermark_19011507_216.jpg

GTX107893 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS34 antibody [C1C3] (GTX107893) diluted at 1:1000.

GTX107893_WB_19011507_630.jpg

GTX107893 WB Image

Sample(30 ug whole cell lysate)
A:A431(GTX27909)
7.5% SDS PAGE
GTX107893 diluted at 1:1000

SDS
Glycerol.pdf
Thimerosal.pdf