Form
Liquid
Buffer
Tris-Citrate/Phosphate
Preservative
0.1% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Mouse
Immunogen
Synthetic peptide conjugated to KLH, derived from a sequence within residues 50-150 of mouse p19ARF. [Swiss-Prot #Q64364]
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
RRID
AB_848642
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Synonyms
CDK2A/p19ARF
Cellular Localization
Cytoplasm,Nucleus,nucleoplasm,Nucleolus
Background
The INK4a/ARF locus encodes two unrelated tumor suppressor proteins, p16INK4a and p19ARF that restrain cell growth by modifying the functions of the retinoblastoma protein and p53, respectively. It is among the most frequently mutated tumor suppressor loci in human cancer. Both p16INK4a and p19ARF act as cell proliferation inhibitors. The ARF gene, p19ARF in mouse and p14ARF in human, has become an important player in cell cycle regulation. In mice, tumor suppressor effects appear to be mediated by interactions between p19ARF and the p53 tumor-suppressor protein. p19ARF counters uncontrolled proliferation and oncogenic signals in p53-dependent pathways. Proteins encoded by the INK4a/ARF locus also play a role in Abelson virus transformation. Both p16INK4a and p19ARF are expressed in many cells as they emerge from the apoptotic crisis that characterizes the transformation process. Expression of p19ARF but not p16INK4a induces apoptosis in Ab-MLV-transformed pre-B cells. INK4a/ARF expression correlates with or precedes the emergence of cells expressing mutant p53. p19ARF is an important part in cellular defense against transforming signals from the Abl oncoprotein, providing direct evidence that the p19ARF-p53 regulatory loop plays an important role in lymphoma induction.
Database
Research Area