APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
0.1-1μg/ml |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX88520-PEP).
Predict Reactivity
Mouse(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Mouse
Immunogen
Peptide with sequence C-EKALQEYRRKSRAE, from the C Terminus of the protein sequence according to NP_038520.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
clusterin , AI893575 , ApoJ , Cli , D14Ucla3 , SP-40 , Sgp-2 , Sgp2 , Sugp-2
Cellular Localization
Secreted , Nucleus , Cytoplasm , Cytoplasm, cytosol , Microsome , Endoplasmic reticulum
Background
The protein encoded by this gene is a secreted chaperone that can, under some stress conditions, also be found in the cell cytosol. It has been suggested to be involved in several basic biological events such as cell death, tumor progression, and neurodegenerative disorders. The encoded preproprotein undergoes proteolytic processing to generate a disulfide-linked heterodimeric mature protein comprised of alpha and beta subunits. Mice lacking the encoded protein exhibit increased severity of autoimmune myocarditis, faster progression of the acute inflammation to myocardial scarring and decreased brain injury following neonatal hypoxic-ischemic injury. [provided by RefSeq, Nov 2015]
Database
Research Area
DATA IMAGES
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GTX88520 WB Image
WB analysis of human testes lysate using GTX88520 Clusterin antibody, C-term. Dilution : 0.3μg/ml Loading : 35μg protein in RIPA buffer
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GTX88520 WB Image
WB analysis of rat brain lysate using GTX88520 Clusterin antibody, C-term. Dilution : 0.1μg/ml Loading : 35μg protein in RIPA buffer
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REFERENCE
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REVIEW
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