APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1 μg/ml |
5-15 μg/ml |
0.25 μg/1 × 10⁶ cells |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 5% Trehalose
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Mouse
Immunogen
recombinant mouse ephrin-B2, expressed in NSO cells.
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
ephrin B2 , ELF-2 , Epl5 , Eplg5 , Htk-L , LERK-5 , Lerk5 , NLERK-1
Cellular Localization
Cell membrane
Background
Ephrin-B2 (the antigen to this antibody) is also known as Htk-L, LERK-5, NLERK-1 and ELF-2 and binds EphA4, EphB1, EphB2, EphB3, and EphB4. Ephrin-B2 can be detected on monocytes, mesangial cells, CD34+ stem cells (ref 1), bone marrow fibroblasts(ref 2), activated melanocytes and melanoma cells (ref 3), and pre-artery endothelial cells (ref 4). To date, at least 14 members of the Eph receptor family and a family of 8 ligands have been identified. Ligands of Eph family receptors are structurally related membrane-bound proteins that can be subdivided into two major subclasses, ephrin-A and ephrin-B. Ligands .
Database
Research Area
REFERENCE
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REVIEW
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