CTP synthase antibody [N1C1]

Name: CTP synthase antibody [N1C1]
Brand: GeneTex
SKU: GTX105265
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-CTP synthase antibody [N1C1] used in Western Blot (WB). GTX105265

Anti-CTP synthase antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105265

Cat. No. GTX105265

Reference 4

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF
Reactivity	Human, Mouse
Package	100 μl ($399), 25 μl ($169)

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View product citations for antibody GTX105265 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	Assay dependent

Not tested in other applications.

Calculated MW

67 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , U87-MG , SK-N-SH , IMR32 , SK-N-AS

Predict Reactivity

Rat, Bovine, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CTP synthase. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036687

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CTP synthase 1 , CTPS , GATD5 , IMD24

Background

The catalytic conversion of UTP to CTP is accomplished by the enzyme cytidine-5-prime-triphosphate synthetase. The enzyme is important in the biosynthesis of phospholipids and nucleic acids, and plays a key role in cell growth, development, and tumorigenesis. The region to which the CTPS gene has been mapped is the location of breakpoints involved in several tumor types [provided by RefSeq]

Database

Gene ID: 1503 CTPS1
UniProt: P17812 CTPS1

Research Area

DATA IMAGES

GTX105265 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CTP synthase antibody [N1C1] (GTX105265) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

GTX105265 WB Image

The data was published in the journal Biol Open in 2014. PMID: 25326513

GTX105265 ICC/IF Image

The data was published in the journal Biol Open in 2014. PMID: 25326513

GTX105265 ICC/IF Image

The data was published in the journal J Cell Sci in 2015.PMID: 26303200

GTX105265 ICC/IF Image

The data was published in the journal J Cell Sci in 2015.PMID: 26303200

REFERENCE

Application Reference

Chang CC et al. Exp Cell Res 2017; (Epub) CTP synthase forms the cytoophidium in human hepatocellular carcinoma.

Application : ICC/IF

Reactivity : Human

Calise SJ et al. J Cell Sci 2016; 129 (15) : 3042-52 'Rod and ring' formation from IMP dehydrogenase is regulated through the one-carbon metabolic pathway.

Application : ICC/IF

Reactivity : Human

Chang CC et al. J Cell Sci 2015; 128 (19) : 602772 Cytoophidium assembly reflects upregulation of IMPDH activity.

Aughey GN et al. Biol Open 2014; 3 (11) : 1045-56 Nucleotide synthesis is regulated by cytoophidium formation during neurodevelopment and adaptive metabolism.

Application : WB

Reactivity : Mouse

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