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PLD3 antibody [N1C1]

Anti-PLD3 antibody [N1C1] used in Western Blot (WB). GTX122218
Anti-PLD3 antibody [N1C1] used in Western Blot (WB). GTX122218

Cat. No. GTX122218

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Drosophila
Package
100 μl ($399),
25 μl ($169)
See all PLD3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Positive Control

A549

Predict Reactivity

Mouse, Rat, Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PLD3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11163961

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

phospholipase D family member 3 , AD19 , HU-K4 , HUK4 , SCA46

Cellular Localization

Endoplasmic reticulum membrane; Single-pass type II membrane protein

Database

Research Area

DATA IMAGES

Anti-PLD3 antibody [N1C1] used in Western Blot (WB). GTX122218

GTX122218 WB Image

Drosophila tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PLD3 antibody [N1C1] (GTX122218) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-PLD3 antibody [N1C1] used in Western Blot (WB). GTX122218

GTX122218 WB Image

Sample (30 ug of whole cell lysate)
A: A549
10% SDS PAGE
GTX122218 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169