APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
0.2-2.5 ug/ml |
2-10 ug/ml |
Not tested in other applications.
Calculated MW
Positive Control
OVCAR-3
PROPERTIES
Form
Liquid
Buffer
PBS, 2% Sucrose
Preservative
0.09% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.5-1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
A synthetic peptide corresponding to a N-terminal region of Human CYP4F3
Purification
Affinity Purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
cytochrome P450 family 4 subfamily F member 3 , CPF3 , CYP4F , LTB4H
Cellular Localization
Endoplasmic reticulum membrane
Background
This gene, CYP4F3, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum. The enzyme starts the process of inactivating and degrading leukotriene B4, a potent mediator of inflammation. This gene is part of a cluster of cytochrome P450 genes on chromosome 19. Another member of this family, CYP4F8, is approximately 18 kb away. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]
Database
Research Area
DATA IMAGES
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GTX47110 IHC-P Image
IHC-P analysis of human liver tissue using GTX47110 CYP4F3 antibody at 1:100. Left to right:DAPI, CYP4F3, Merge. Low pH, heat-induced antigen retrieval method utilizing Sodium Citrate buffer was performed.
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GTX47110 WB Image
WB analysis of OVCAR-3 cells using GTX47110 CYP4F3 antibody at 0.2-1μg/ml.
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REFERENCE
There are currently no references for CYP4F3 antibody, N-term (GTX47110). Be the first to share your publications with this product.
REVIEW
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