Form
Liquid
Buffer
20mM Tris-HCl, 150mM NaCl, 20% Glycerol, 1mM DTT
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.5 mg/ml (Please refer to the vial label for the specific concentration.)
Region/Sequence
Full length protein, N-terminal His-Tag; MGSSHHHHHH SSGLVPRGSH MGSMQPGSSR CEEETPSLLW GLDPVFLAFA KLYIRDILDM KESRQVPGVF LYNGHPIKQV DVLGTVIGVR ERDAFYSYGV DDSTGVINCI CWKKLNTESV SAAPSAAREL SLTSQLKKLQ ETIEQKTKIE IGDTIRVRGS IRTYREEREI HATAYYKVDD PVWNIQIARM LELPTIYRKV YDQPFHSSAL EKEEALSNPG ALDLPSLTSL LSEKAKEFLM ENRVQSFYQQ ELEMVESLLS LANQPVIHSA CSDQVNFKKD TTSKAIHSIF KNAIQLLQEK GLVFQKDDGF DNLYYVTRED KDLHRKIHRI IQQDCQKPNH MEKGCHFLHI LACARLSIRP GLSEAVLQQV LELLEDQSDI VSTMEHYYTA F
Expression System
E. coli
Purity
> 85% by SDS-PAGE.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Synonyms
STN1 subunit of CST complex , AAF-44 , AAF44 , OBFC1 , RPA-32 , bA541N10.2
Cellular Localization
Nucleus
Background
OBFC1 and C17ORF68 (MIM 613129) are subunits of an alpha accessory factor (AAF) that stimulates the activity of DNA polymerase-alpha-primase (see MIM 176636), the enzyme that initiates DNA replication (Casteel et al., 2009 [PubMed 19119139]). OBFC1 also appears to function in a telomere-associated complex with C17ORF68 and TEN1 (C17ORF106; MIM 613130) (Miyake et al., 2009 [PubMed 19854130]).[supplied by OMIM, Nov 2009]
Database
Research Area