PRODUCT
Summary
GAPDH antibody recognizes glyceraldehyde-3-phosphate dehydrogenase (GAPDH) protein, a glycolytic enzyme of ~37 kDa that forms a tetramer. Because of its widespread expression, GAPDH is commonly used as an internal control for various protein assays, particularly western blot, to validate both the amount and quality of the loaded lysate samples.
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:5000-1:100000 |
1:100-1:1000 |
1:100-1:1000 |
1:100-1:500 |
Not tested in other applications.
Calculated MW
Positive Control
PC-12 , Rat2 , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , E.coli M15
Predict Reactivity
Sheep, Cat, Guinea pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 1% BSA, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.08 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human GAPDH. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1080976
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
glyceraldehyde-3-phosphate dehydrogenase , G3PD , GAPD , HEL-S-162eP
Cellular Localization
Cytoplasm , perinuclear region , Membrane
Background
The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. [provided by RefSeq]
Database
Research Area
DATA IMAGES
|
GTX100118 ICC/IF Image
GAPDH antibody detects GAPDH protein at endoplasmic reticulum, cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: GAPDH stained by GAPDH antibody (GTX100118) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
|
|
GTX100118 IHC-P Image
GAPDH antibody detects GAPDH protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded human duodenum.GAPDH stained by GAPDH antibody (GTX100118) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
GTX100118 WB Image
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody (GTX100118) diluted at 1:5000.
|
|
GTX100118 IP Image
Immunoprecipitation of GAPDH protein from 293T whole cell extracts using 5 μg of GAPDH antibody (GTX100118). Western blot analysis was performed using GAPDH antibody (GTX100118). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
|
|
GTX100118 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody (GTX100118) diluted at 1:6000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
GTX100118 IHC-P Image
GAPDH antibody detects GAPDH protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse intestine.GAPDH stained by GAPDH antibody (GTX100118) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
GTX100118 IHC-P Image
GAPDH antibody detects GAPDH protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat testis.GAPDH stained by GAPDH antibody (GTX100118) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
GTX100118 IHC-P Image
GAPDH antibody detects GAPDH protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse liver.GAPDH stained by GAPDH antibody (GTX100118) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
GTX100118 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody (GTX100118) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
GTX100118 WB Image
Whole cell extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody (GTX100118) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
GTX100118 IHC-P Image
GAPDH antibody detects GAPDH protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse duodenum.GAPDH stained by GAPDH antibody (GTX100118) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
GTX100118 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody (GTX100118) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
GTX100118 IHC-P Image
GAPDH antibody detects GAPDH protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat liver.GAPDH stained by GAPDH antibody (GTX100118) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
GTX100118 WB Image
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody (GTX100118) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
GTX100118 WB Image
Various whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody (GTX100118) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
GTX100118 WB Image
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody (GTX100118) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
GTX100118 WB Image
The data was published in the 2021 in Cancer Drug Resist. PMID: 35582384
|
|
GTX100118 WB Image
The data was published in the 2022 in Front Pharmacol. PMID: 35517780
|
|
GTX100118 WB Image
The data was published in the journal Cell Death Differ in 2019. PMID: 30237510
|
|
GTX100118 WB Image
The data was published in the journal PLoS Pathog in 2015. PMID: 25816318
|
|
GTX100118 WB Image
The data was published in the journal Sci Rep in 2017. PMID: 28216671
|
|
GTX100118 WB Image
The data was published in the 2022 in Pharmaceutics. PMID: 35456673
|
|
GTX100118 WB Image
The data was published in the 2022 in Front Pharmacol. PMID: 35517780
|
|
GTX100118 WB Image
The data was published in the 2022 in Front Microbiol. PMID: 35572621
|
|
GTX100118 WB Image
The data was published in the 2022 in Front Pharmacol. PMID: 35517780
|
|
GTX100118 WB Image
The data was published in the 2022 in Front Pharmacol. PMID: 35517780
|
|
GTX100118 WB Image
The data was published in the 2022 in Front Microbiol. PMID: 35572621
|
|
GTX100118 WB Image
The data was published in the 2022 in Cell Death Dis. PMID: 36435842
|
|
GTX100118 WB Image
The data was published in the 2022 in Cell Death Dis. PMID: 35688824
|
|
GTX100118 WB Image
The data was published in the 2022 in Front Microbiol. PMID: 35572621
|
|
GTX100118 WB Image
The data was published in the 2021 in Cancer Cell Int. PMID: 33926470
|
|
GTX100118 WB Image
The data was published in the 2021 in Stem Cell Res Ther. PMID: 34020702
|
|
GTX100118 WB Image
The data was published in the 2022 in Biomedicines. PMID: 35203455
|
|
GTX100118 WB Image
The data was published in the 2021 in Eur Psychiatry. PMID: 33413711
|
|
GTX100118 WB Image
The data was published in the journal Int J Mol Sci in 2020.PMID: 32098449
|
|
GTX100118 WB Image
The data was published in the journal Sci Rep in 2017.PMID: 28098200
|
|
GTX100118 WB Image
The data was published in the journal Front Aging Neurosci in 2014.PMID: 25136316
|
|
GTX100118 WB Image
The data was published in the journal PLoS Biol in 2015.PMID: 26406915
|
|
GTX100118 WB Image
The data was published in the journal PLoS Biol in 2015.PMID: 26406915
|
|
GTX100118 WB Image
The data was published in the journal Oncotarget in 2015.PMID: 25797272
|
|
GTX100118 WB Image
The data was published in the journal Int J Mol Sci in 2020.PMID: 32545770
|
|
GTX100118 WB Image
The data was published in the journal Sci Rep in 2020.PMID: 32427884
|
|
GTX100118 WB Image
The data was published in the journal Stem Cell Res Ther in 2018.PMID: 29751772
|
|
GTX100118 WB Image
The data was published in the journal BMC Cancer in 2018.PMID: 30594165
|
|
GTX100118 WB Image
The data was published in the journal Oncotarget in 2015.PMID: 25797272
|
|
GTX100118 WB Image
The data was published in the journal PLoS One in 2016.PMID: 27023634
|
|
GTX100118 WB Image
The data was published in the journal Stem Cell Res Ther in 2018.PMID: 29751772
|
|
GTX100118 WB Image
The data was published in the journal Mol Oncol in 2020.PMID: 32037723
|
|
GTX100118 WB Image
The data was published in the journal Sci Rep in 2017.PMID: 28098200
|
|
GTX100118 WB Image
The data was published in the journal Nat Commun in 2020.PMID: 32286300
|
|
GTX100118 WB Image
The data was published in the journal Sci Rep in 2017.PMID: 28098200
|
|
GTX100118 WB Image
The data was published in the journal Int J Mol Sci in 2020.PMID: 32545770
|
|
GTX100118 WB Image
The data was published in the journal Int J Mol Sci in 2020.PMID: 32545770
|
|
GTX100118 WB Image
The data was published in the journal PLoS One in 2016.PMID: 27023634
|
|
GTX100118 WB Image
The data was published in the journal Sci Rep in 2017.PMID: 28098200
|
|
GTX100118 WB Image
The data was published in the journal PLoS One in 2016.PMID: 27023634
|
|
GTX100118 WB Image
The data was published in the journal Oncotarget in 2016.PMID: 27564110
|
|
GTX100118 WB Image
The data was published in the journal PLoS One in 2016.PMID: 27023634
|
|
GTX100118 WB Image
The data was published in the journal Stem Cell Res Ther in 2018.PMID: 29751772
|
|
GTX100118 WB Image
The data was published in the journal Cell Death Dis in 2019.PMID: 31427568
|
|
GTX100118 WB Image
The data was published in the journal PLoS One in 2016.PMID: 27383761
|
|
GTX100118 WB Image
The data was published in the journal Nat Commun in 2020.PMID: 32286300
|
|
GTX100118 WB Image
The data was published in the journal PLoS One in 2016.PMID: 27023634
|
|
GTX100118 WB Image
The data was published in the journal PLoS One in 2019.PMID: 30893332
|
|
GTX100118 WB Image
The data was published in the journal PLoS Biol in 2015.PMID: 26406915
|
|
GTX100118 WB Image
The data was published in the journal J Virol in 2016.PMID: 27489281
|
|
GTX100118 WB Image
The data was published in the journal PLoS One in 2016.PMID: 27023634
|
|
GTX100118 WB Image
The data was published in the journal Stem Cell Res Ther in 2018.PMID: 29751772
|
|
GTX100118 WB Image
The data was published in the journal Front Immunol in 2020.PMID: 32210961
|
|
GTX100118 WB Image
The data was published in the journal Physiol Rep in 2016. PMID: 27482075
|
|
GTX100118 WB Image
The data was published in the journal PLoS One in 2015. PMID: 25938237
|
|
GTX100118 WB Image
The data was published in the journal PLoS One in 2015. PMID: 25938237
|
|
GTX100118 WB Image
The data was published in the journal Front Physiol in 2016. PMID: 27965586
|
|
GTX100118 WB Image
The data was published in the journal J Biomed Sci in 2013. PMID: 24359494
|
|
GTX100118 WB Image
The data was published in the journal PLoS One in 2016. PMID: 26863132
|
|
GTX100118 WB Image
The data was published in the journal Front Physiol in 2016. PMID: 27965586
|
|
GTX100118 WB Image
The data was published in the journal Evid Based Complement Alternat Med in 2013. PMID: 23573126
|
|
GTX100118 WB Image
The data was published in the journal PLoS One in 2015. PMID: 25799508
|
|
GTX100118 WB Image
The data was published in the journal PLoS Genet in 2015. PMID: 25760041
|
|
GTX100118 WB Image
The data was published in the journal J Immunol Res in 2017. PMID: 29423418
|
|
GTX100118 WB Image
The data was published in the journal Front Microbiol in 2019. PMID: 31969874
|
|
GTX100118 WB Image
The data was published in the journal Brain Behav in 2013. PMID: 24363972
|
|
GTX100118 WB Image
The data was published in the journal Virulence in 2018. PMID: 29338543
|
|
GTX100118 WB Image
The data was published in the journal Oncotarget in 2016. PMID: 27191263
|
|
GTX100118 WB Image
The data was published in the journal Virulence in 2018. PMID: 29338543
|
|
GTX100118 WB Image
The data was published in the journal Cancers (Basel) in 2019. PMID: 30634502
|
|
GTX100118 WB Image
The data was published in the journal Virulence in 2018. PMID: 29338543
|
|
GTX100118 WB Image
The data was published in the journal PLoS One in 2015. PMID: 26360058
|
|
GTX100118 WB Image
The data was published in the journal PLoS One in 2015. PMID: 26360058
|
|
GTX100118 WB Image
The data was published in the journal J Hematol Oncol in 2018. PMID: 29458389
|
|
GTX100118 WB Image
The data was published in the journal J Cell Mol Med in 2014. PMID: 24400868
|
|
GTX100118 WB Image
The data was published in the journal Evid Based Complement Alternat Med in 2012. PMID: 23024692
|
|
GTX100118 WB Image
The data was published in the journal PLoS One in 2015. PMID: 26360058
|
|
GTX100118 WB Image
The data was published in the journal J Cell Mol Med in 2014. PMID: 24400868
|
|
GTX100118 WB Image
The data was published in the journal J Cell Mol Med in 2014. PMID: 24400868
|
|
GTX100118 WB Image
The data was published in the journal J Biomed Sci in 2013. PMID: 24359494
|
|
GTX100118 WB Image
The data was published in the journal Dis Model Mech in 2014. PMID: 24271779
|
|
GTX100118 WB Image
The data was published in the journal J Biomed Sci in 2014. PMID: 25200553
|
|
GTX100118 WB Image
The data was published in the journal Dis Model Mech in 2014. PMID: 24271779
|
|
GTX100118 WB Image
The data was published in the journal Dis Model Mech in 2014. PMID: 24271779
|
|
GTX100118 WB Image
The data was published in the journal Cell Discov in 2015. PMID: 27462424
|
|
GTX100118 WB Image
The data was published in the journal Cell Discov in 2015. PMID: 27462424
|
|
GTX100118 WB Image
The data was published in the journal Evid Based Complement Alternat Med in 2013. PMID: 23573126
|
|
GTX100118 WB Image
The data was published in the journal J Biomed Sci in 2014. PMID: 25200553
|
|
GTX100118 WB Image
The data was published in the journal J Biomed Sci in 2014. PMID: 25200553
|
|
GTX100118 WB Image
The data was published in the journal Evid Based Complement Alternat Med in 2013. PMID: 24282433
|
|
GTX100118 WB IP Image
The data was published in the journal J Hematol Oncol in 2019. PMID: 31324208
|
|
GTX100118 WB Image
The data was published in the journal PLoS Negl Trop Dis in 2018. PMID: 29505577
|
|
GTX100118 WB Image
The data was published in the journal mSphere in 2019. PMID: 30814317
|
|
GTX100118 WB Image
The data was published in the journal Oncotarget in 2018. PMID: 29899821
|
|
GTX100118 WB Image
The data was published in the journal Oncotarget in 2016. PMID: 27034167
|
|
GTX100118 WB Image
The data was published in the journal Oncotarget in 2016. PMID: 27191263
|
|
GTX100118 WB Image
The data was published in the journal Oncotarget in 2016. PMID: 27034167
|
|
GTX100118 WB Image
The data was published in the journal J Immunol Res in 2018. PMID: 30410941
|
|
GTX100118 WB Image
The data was published in the journal J Cell Mol Med in 2014. PMID: 24400868
|
|
GTX100118 WB Image
The data was published in the journal PLoS One in 2014. PMID: 24551118
|
|
GTX100118 WB Image
The data was published in the journal PLoS One in 2015. PMID: 26360058
|
|
GTX100118 WB Image
The data was published in the journal PLoS One in 2014. PMID: 24551118
|
|
GTX100118 WB Image
The data was published in the journal Biomolecules in 2019. PMID: 31443530
|
|
GTX100118 WB Image
The data was published in the journal Breast Cancer Res in 2017. PMID: 29258605
|
|
GTX100118 WB Image
The data was published in the journal Biomolecules in 2019. PMID: 31443530
|
|
GTX100118 WB Image
The data was published in the journal Biomolecules in 2019. PMID: 31443530
|
|
GTX100118 WB Image
The data was published in the journal Cell Death Dis in 2013. PMID: 23788038
|
|
GTX100118 WB Image
The data was published in the journal Biomolecules in 2019. PMID: 31443530
|
|
GTX100118 WB Image
The data was published in the journal Biomolecules in 2019. PMID: 31443530
|
|
GTX100118 WB Image
The data was published in the journal Oncotarget in 2016. PMID: 27191263
|
|
GTX100118 WB Image
The data was published in the journal Virulence in 2018. PMID: 29338543
|
|
GTX100118 WB Image
The data was published in the journal PLoS Pathog in 2019. PMID: 30742689
|
|
GTX100118 WB Image
The data was published in the journal Cell Div in 2019. PMID: 31110557
|
|
GTX100118 WB Image
The data was published in the journal Cancers (Basel) in 2019. PMID: 30634502
|
|
GTX100118 WB Image
The data was published in the journal Oncotarget in 2018. PMID: 29899821
|
|
GTX100118 WB Image
The data was published in the journal PLoS One in 2015. PMID: 25738497
|
|
GTX100118 WB Image
The data was published in the journal J Cell Mol Med in 2020. PMID: 31821701
|
|
GTX100118 WB Image
The data was published in the journal Oncotarget in 2018. PMID: 29899821
|
|
GTX100118 WB Image
The data was published in the journal Oncotarget in 2018. PMID: 29899821
|
|
GTX100118 WB Image
The data was published in the journal PLoS One in 2015. PMID: 25928539
|
|
GTX100118 WB Image
The data was published in the journal Biomolecules in 2019. PMID: 31505885
|
|
GTX100118 WB Image
The data was published in the journal Oncotarget in 2016. PMID: 27034167
|
|
GTX100118 WB Image
The data was published in the journal Cell Adh Migr in 2019. PMID: 31240993
|
|
GTX100118 WB Image
The data was published in the journal PLoS One in 2015. PMID: 25811469
|
|
GTX100118 WB Image
The data was published in the journal PLoS One in 2015. PMID: 25811469
|
|
GTX100118 WB Image
The data was published in the journal Oncotarget in 2018. PMID: 29899821
|
|
GTX100118 WB Image
The data was published in the journal PLoS One in 2015. PMID: 25738497
|
|
GTX100118 WB Image
The data was published in the journal Acta Neuropathol in 2019. PMID: 30874922
|
|
GTX100118 WB Image
The data was published in the journal Front Endocrinol (Lausanne) in 2017. PMID: 29109696
|
|
GTX100118 WB Image
The data was published in the journal PLoS One in 2015. PMID: 25738497
|
|
GTX100118 WB Image
The data was published in the journal Front Immunol in 2020.PMID: 32210961
|
|
GTX100118 WB Image
The data was published in the journal Front Immunol in 2020.PMID: 32210961
|
|
GTX100118 WB Image
The data was published in the journal Stem Cells Transl Med in 2020.PMID: 31943851
|
|
GTX100118 WB Image
The data was published in the journal Front Immunol in 2020.PMID: 32210961
|
|
GTX100118 WB Image
The data was published in the journal Sci Rep in 2017.PMID: 28098200
|
|
GTX100118 WB Image
The data was published in the journal Stem Cells Transl Med in 2020.PMID: 31943851
|
|
GTX100118 WB Image
The data was published in the journal PLoS One in 2015. PMID: 25938237
|
|
GTX100118 WB Image
The data was published in the 2022 in Int J Mol Sci. PMID: 35328622
|
|
GTX100118 WB Image
The data was published in the 2019 in Cell Div. PMID: 31452676
|
|
GTX100118 WB Image
The data was published in the 2020 in Sci Rep. PMID: 33177556
|
|
GTX100118 WB Image
The data was published in the 2019 in Cell Div. PMID: 31452676
|
|
GTX100118 WB Image
The data was published in the 2019 in Cell Div. PMID: 31452676
|
|
GTX100118 WB Image
The data was published in the 2014 in Alzheimers Res Ther. PMID: 25478031
|
|
GTX100118 WB Image
The data was published in the journal PLoS One in 2015. PMID: 26305546
|
|
GTX100118 WB Image
The data was published in the 2020 in Sci Rep. PMID: 32424165
|
|
GTX100118 WB Image
The data was published in the 2021 in Cancer Cell Int. PMID: 33926470
|
|
GTX100118 WB Image
The data was published in the 2019 in Oncogene. PMID: 30792539
|
|
GTX100118 WB Image
The data was published in the 2019 in Oncogene. PMID: 30792539
|
|
GTX100118 WB Image
The data was published in the 2022 in FEBS Open Bio. PMID: 34856073
|
|
GTX100118 WB Image
The data was published in the 2019 in Oncogene. PMID: 30792539
|
|
GTX100118 WB Image
The data was published in the 2022 in Front Cell Dev Biol. PMID: 35309907
|
|
GTX100118 WB Image
The data was published in the journal Oncoscience in 2016. PMID: 28105458
|
|
GTX100118 WB Image
The data was published in the journal PLoS Pathog in 2019. PMID: 30742689
|
|
GTX100118 WB Image
The data was published in the journal Oncogenesis in 2015. PMID: 26690546
|
|
GTX100118 WB Image
The data was published in the journal PLoS One in 2015. PMID: 25876076
|
|
GTX100118 WB Image
The data was published in the journal PLoS Pathog in 2019. PMID: 30742689
|
|
GTX100118 WB Image
The data was published in the journal Oncotarget in 2018. PMID: 29899821
|
|
GTX100118 WB Image
The data was published in the journal Int J Mol Sci in 2018. PMID: 29518977
|
|
GTX100118 WB Image
The data was published in the journal PLoS One in 2015. PMID: 25811469
|
|
GTX100118 WB Image
The data was published in the journal Oncotarget in 2018. PMID: 29899821
|
|
GTX100118 WB Image
The data was published in the journal PLoS One in 2015. PMID: 25928539
|
|
GTX100118 WB Image
The data was published in the journal Cell Adh Migr in 2019. PMID: 31240993
|
|
GTX100118 WB Image
The data was published in the journal J Cell Mol Med in 2020. PMID: 31821701
|
|
GTX100118 WB Image
The data was published in the journal Cell Death Dis in 2013. PMID: 23788038
|
|
GTX100118 WB Image
The data was published in the journal J Int Soc Sports Nutr in 2015. PMID: 25792976
|
|
GTX100118 WB Image
The data was published in the journal Biomolecules in 2019. PMID: 31443530
|
|
GTX100118 WB Image
The data was published in the journal Int J Mol Sci in 2016. PMID: 26938532
|
|
GTX100118 WB Image
The data was published in the journal Nat Commun in 2020. PMID: 32358491
|
|
GTX100118 WB Image
The data was published in the journal Front Endocrinol (Lausanne) in 2017. PMID: 29109696
|
|
GTX100118 WB Image
The data was published in the journal Oncogenesis in 2015. PMID: 26690546
|
|
GTX100118 WB Image
The data was published in the journal Aging Cell in 2020. PMID: 31833196
|
|
GTX100118 WB Image
The data was published in the journal Oncogenesis in 2015. PMID: 26690546
|
|
GTX100118 WB Image
The data was published in the journal Int J Mol Sci in 2016. PMID: 26938532
|
REFERENCE
REVIEW
GAPDH antibody
Cat. No. GTX100118
Rating
|
|
( Average 4.6 based on 19 users reviews)
|
Application
Western Blot(WB)
|
|
( Average 4.6 based on 18 users reviews)
|
Immunocytochemistry/ Immunofluorescence(ICC/IF)
|
|
( Average 5 based on 1 users reviews)
|
Date :
Anonymous submitted on 22-Sep-2023
Application Tested :
WB
Sample Species :
Hu, Ms
Sample :
HEK293T cells, NIH3T3 cells
Amount used :
30 μg
Blocking :
5% nonfat-skim milk in TBST, 25°C, 1Hr
Primary Antibody :
1:5000, 4°C, 17Hr
Date :
Anonymous submitted on 12-Jul-2023
Application Tested :
WB
Sample Species :
Ms
Sample :
mouse liver tissue lysate (ICR)
Amount used :
40μg
Blocking :
5% non-fat milk in TBST, 25°C, 1Hr
Primary Antibody :
Undiluted, 4°C, >12Hr
Date :
Anonymous submitted on 30-Mar-2021
Application Tested :
WB
Sample Species :
Hu
Sample :
OC-3-VGH cells
Amount used :
35 μg
Blocking :
5% non-fat milk in TBST, 4°C, 20Hr
Primary Antibody :
1:5000, 4°C, 20Hr
Date :
Anonymous submitted on 13-Aug-2018
Application Tested :
WB
Sample Species :
Hu
Sample :
SAS cells
Amount used :
29 μg
Blocking :
BSA, 25°C, 1Hr
Primary Antibody :
1:5000, 4°C, 16Hr
Date :
Anonymous submitted on 31-Jul-2017
Application Tested :
WB
Sample Species :
Hu
Sample :
A549 cells
Amount used :
30 μg
Blocking :
5% skim milk, 25°C, 1Hr
Primary Antibody :
1:5000, 25°C, 2Hr
Date :
Anonymous submitted on 29-Apr-2016
Application Tested :
WB
Sample Species :
Ms
Sample :
Brown adipose tissue
Amount used :
32μg
Blocking :
5% milk, 25°C, 1Hr
Primary Antibody :
1:1000, 4°C, 16Hr
Date :
Anonymous submitted on 07-Apr-2015
Application Tested :
WB
Sample Species :
Ms
Sample :
skin tissue
Amount used :
20μg
Blocking :
5% non-fat milk, 25°C, 2Hr
Primary Antibody :
1:10000, 4°C, 16Hr
Date :
Anonymous submitted on 27-Jan-2015
Application Tested :
WB
Sample Species :
Rat
Sample :
A7R5 cells, H9C2 cells
Amount used :
40μg
Blocking :
3% BSA in TBST, 25°C, 1Hr
Primary Antibody :
1:10000, 4°C, 24Hr
Lane Description :
Lane1: A7R5 cells Lane2: H9C2 cells
Date :
Anonymous submitted on 09-Oct-2014
Application Tested :
WB
Sample Species :
Hu
Sample :
U2OS cells
Amount used :
2μg
Blocking :
5% milk in 0.1%TBST, 25°C, 0.5Hr
Primary Antibody :
1:20000, 25°C, 1Hr
Date :
Anonymous submitted on 07-Aug-2014
Application Tested :
WB
Sample Species :
Hu
Sample :
human lung cancer cells
Amount used :
30μg
Blocking :
25°C, 2Hr
Primary Antibody :
1:10000, 4°C, 10Hr
Date :
Anonymous submitted on 09-May-2014
Application Tested :
WB
Sample Species :
Hu
Sample :
293T cells
Amount used :
28μg
Blocking :
5% BSA in TBST, 4°C, 1Hr
Primary Antibody :
1:5000, 4°C, 14Hr
Date :
Anonymous submitted on 05-Jun-2013
Application Tested :
WB
Sample Species :
Hu
Sample :
A549 cells,HCC827 cells,H1975 cells,H358 cells
Amount used :
50μg
Blocking :
5% milk, 25°C, 1Hr
Primary Antibody :
1:5000, 25°C, 1Hr
Date :
Anonymous submitted on 16-Nov-2012
Application Tested :
WB
Sample Species :
Hu
Sample :
HEK293T cells
Amount used :
30μg
Blocking :
1% BSA/TBST, 2Hr
Primary Antibody :
1:1000, 4°C, 16Hr
Date :
Anonymous submitted on 24-Aug-2012
Application Tested :
ICC/IF
Sample Species :
Hu
Sample :
HEK293 cells
Blocking :
1% BSA, 37°C, 0.5Hr
Fixation :
4% paraformaldehyde
Permeabilization /Antigen retrieval :
0.1% Triton X-100
Primary Antibody :
1:500, 37°C, 1Hr
Date :
Anonymous submitted on 24-Aug-2012
Application Tested :
WB
Sample :
Drosophila eye disc tissue
Blocking :
5% milk in PBST, 25°C, 1Hr
Primary Antibody :
1:3000, 4°C, 16Hr
Date :
Anonymous submitted on 30-Jan-2012
Application Tested :
WB
Sample Species :
Ms
Sample :
Mouse cerebral endothelial tissue
Amount used :
30μg
Blocking :
5% skim milk in TBST, 4°C, overnightHr
Primary Antibody :
1:5000, 25°C, 2Hr
Date :
Anonymous submitted on 16-Jan-2012
Application Tested :
WB
Sample Species :
Hu
Sample :
SH-SY5Y cells
Amount used :
25μg
Blocking :
5% non-fat milk, 1Hr
Primary Antibody :
1:1000, 4°C, 16Hr
Date :
Anonymous submitted on 16-Jan-2012
Application Tested :
WB
Sample Species :
Hu
Sample :
A431 cells
Amount used :
30μg
Blocking :
5% BSA, 25°C, 1Hr
Primary Antibody :
1:60000, 4°C, 16Hr
Date :
Anonymous submitted on 31-Oct-2011
Application Tested :
WB
Sample Species :
Hu
Sample :
A549 cells
Amount used :
10μg
Blocking :
5% milk in TBST, 25°C, 2Hr
Primary Antibody :
1:1000, 25°C, 2Hr