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The SARS-CoV-2 Omicron Variant

08-Aug-2022

On 26 November 2021, the WHO designated the SARS-CoV-2 variant B.1.1.529 to be the fifth Variant of Concern (VOC) and assigned it the name “Omicron”. Of the more than 50 mutations in its genome, at least 32 are in the spike protein with fifteen point mutations in the RBD (Figure 1), including several found in other VOCs linked to increased transmissibility and immune evasion. Like other VOCs, mutations are also found in Omicron’s nucleocapsid protein (Figure 2). Omicron is widely distributed and is outcompeting Delta. Though it appears to cause more mild disease, it nevertheless has driven increased hospitalizations.

The SARS-CoV-2 Omicron Variant Spike Mutation Sites

Figure 1. Spike mutation sites in Omicron

 

SARS-CoV-2 Omicron Variant Nucleocapsid Mutation Sites

Figure 2. Nucleocapsid protein mutation sites in Omicron

 

GeneTex has a wide range of recombinant antibody and protein reagents for Omicron research. Notably, nucleocapsid antibodies whose immunogens do not contain any Omicron mutation sites are shown in Figure 2.

 

Targeted Region of Spike
Antibody Cat. No.
Clone
 BA.1
(GTX136780-pro)		 BA.2
(GTX137097-pro)		 BA.2.12.1
(GTX137114-pro)		 BA.4 / BA.5
(GTX137113-pro)
RBD GTX635807 HL1014 ● ● ● ●
RBD GTX635792 HL1003 ● ○ ● X
RBD GTX635793 HL1004 X ○ ○ ●
S2 GTX632604 1A9 ○ ○ ● ●
S2 GTX635693 HL237 ● ● ● ●

 

Recombinant spike proteins (trimer) derived from different SARS-CoV-2 variants were assayed by indirect ELISA using the specified GeneTex monoclonal antibodies.

●   Strong detection. Defined as OD450 value >1 when 1μg/ml protein was used.
○   Weak detection. Defined as OD450 value >1 when 4μg/ml protein was used.
X   No detection. Defined as OD450 value <1 when 4μg/ml protein was used.

 

Highlighted Products
  
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BA.4 / BA.5 variant, His tag

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BA.4 / BA.5 variant, His tag
(GTX137113-pro)
SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BA.4 / BA.5 variant, His tag

SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BA.4 / BA.5 variant, His tag
(GTX137098-pro)
SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014]

SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014]
(GTX635807)
SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004]

SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004]
(GTX635793)
Lateral Flow Assay (Recommended Nucleocapsid Antibody Pairs)
Recombinant Abs Citation Support Orthogonal Validation

 

Antibody Pair   Product Name Clonality EC50 Cat. No.
Pair I   Orthogonal Validation Nucleocapsid antibody [HL5511] Rb recAb 14 pM GTX635689
 Orthogonal Validation Nucleocapsid antibody [HL448] Rb recAb 11 pM GTX635686
Pair II  Orthogonal Validation Nucleocapsid antibody [HL5410] Rb recAb 9 pM GTX635685
 Orthogonal Validation Nucleocapsid antibody [HL455] Rb recAb 6 pM GTX635688

 

 

Omicron Spike and Nucleocapsid Recombinant Proteins
 
Product Name Expression System Cat. No.
SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BA.2.75 variant, His tag HEK293 cells GTX137422-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BA.2.75 variant, His tag HEK293 cells GTX137533-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BA.1 variant, His tag HEK293 cells GTX136780-pro
SARS-CoV-2 (COVID-19) Nucleocapsid protein, Omicron / BA.1 variant, His tag HEK293 cells GTX136779-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BA.2 variant, His tag HEK293 cells GTX137037-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BA.4 / BA.5 variant, His tag HEK293 cells GTX137113-pro
SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BA.2 variant, His tag HEK293 cells GTX136905-pro
SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BA.1 variant, His tag HEK293 cells GTX136716-pro
SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BA.4 / BA.5 variant, His tag HEK293 cells GTX137098-pro
SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BA.2.12.1 variant, His tag HEK293 cells GTX137249-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BA.2.12.1 variant, His tag HEK293 cells GTX137114-pro
SARS-CoV-2 (COVID-19) Nucleocapsid protein, Omicron / BA.2 / BA.5 variant, His tag HEK293 cells GTX137219-pro
SARS-CoV-2 (COVID-19) Nucleocapsid protein, B.1.1.529 / Omicron variant, His tag E. coli GTX03400-pro

 

 

Reagents that detect Omicron
Recombinant Abs Citation Support  Orthogonal Validation

 

Product Name Applications Cat. No.  
SARS-CoV-2 (COVID-19) Nucleocapsid ELISA Pair [HL5511 / HL448] ELISA, Sandwich ELISA GTX500045  
SARS-CoV-2 (COVID-19) Spike RBD ELISA Pair [HL1014 / HL1003] ELISA, Sandwich ELISA GTX500046  
SARS-CoV-2 (COVID-19) Spike RBD Protein Sandwich ELISA Kit ELISA, Sandwich ELISA GTX536267  
SARS-CoV-2 (COVID-19) Spike-ACE2 Binding / Neutralization Assay Kit (Omicron BA.1 / BA.2) Neutralizing/Inhibition GTX537054  Orthogonal Validation
SARS-CoV-2 (COVID-19) Spike RBD antibody WB, ICC/IF, FACS, ELISA, Neutralizing/Inhibition, Sandwich ELISA, IHC-P (cell pellet) GTX135709  
SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] WB, ICC/IF, IHC-P, FACS, IP, ELISA, EM, Sandwich ELISA, IHC-P (cell pellet) GTX632604  
SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL344] WB, ICC/IF, IHC-P, IHC-Fr, ELISA, Sandwich ELISA, IHC-P (cell pellet) GTX635679  Recombinant Abs Orthogonal Validation
SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5410] WB, ICC/IF, ELISA, Lateral Flow, Sandwich ELISA, IHC-P (cell pellet) GTX635685  Recombinant Abs Orthogonal Validation
SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL448] WB, ICC/IF, IHC-P, ELISA, Lateral Flow, Sandwich ELISA, IHC-P (cell pellet) GTX635686  Recombinant Abs Citation Support Orthogonal Validation
SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL448] (HRP) WB, ELISA, Sandwich ELISA GTX635686-01  Recombinant Abs Citation Support
SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL453] WB, ICC/IF, ELISA, IHC-P (cell pellet) GTX635687  Recombinant Abs Orthogonal Validation
SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL455] WB, ICC/IF, ELISA, Lateral Flow, Sandwich ELISA, IHC-P (cell pellet) GTX635688  Recombinant Abs Orthogonal Validation
SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] WB, ICC/IF, ELISA, Lateral Flow, Sandwich ELISA, IHC-P (cell pellet) GTX635689  Recombinant Abs Citation Support Orthogonal Validation
SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] WB, ICC/IF, IHC-P, ELISA, IHC-P (cell pellet) GTX635692  Recombinant Abs
SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] WB, ICC/IF, ELISA, Sandwich ELISA, IHC-P (cell pellet) GTX635693  Recombinant Abs 
SARS-CoV-2 (COVID-19) Spike S1 antibody [GT263] WB, ICC/IF, ELISA, Sandwich ELISA, IHC-P (cell pellet) GTX635708  
SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL455-MS] WB, ICC/IF, ELISA, Sandwich ELISA, IHC-P (cell pellet) GTX635712  Recombinant Abs Citation Support Orthogonal Validation
SARS-CoV-2 (COVID-19) Spike S1 antibody [HL13402] WB, ICC/IF, ELISA, Sandwich ELISA GTX635713  Recombinant Abs 
SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] ELISA, Lateral Flow, Neutralizing/Inhibition, Sandwich ELISA GTX635791  Recombinant Abs Citation Support
SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] ICC/IF, ELISA, Neutralizing/Inhibition, Sandwich ELISA GTX635792  Recombinant Abs Citation Support 
SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (HRP) ELISA, Sandwich ELISA GTX635792-01  Recombinant Abs 
SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] ICC/IF, ELISA, Neutralizing/Inhibition, Sandwich ELISA GTX635793  Recombinant Abs
SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (HRP) ELISA, Sandwich ELISA GTX635793-01  Recombinant Abs
SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] ICC/IF, ELISA, Sandwich ELISA GTX635807  Recombinant Abs 
SARS-CoV-2 (COVID-19) Nucleocapsid antibody [GT113] WB, ICC/IF, ELISA, Sandwich ELISA GTX635808  Orthogonal Validation
SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] WB, ICC/IF, ELISA, Sandwich ELISA, IHC-P (cell pellet) GTX635910  Recombinant Abs
SARS-CoV-2 (COVID-19) Spike S2 / S2' antibody [HL1039] WB, ICC/IF, ELISA, Sandwich ELISA, IHC-P (cell pellet) GTX635911  Recombinant Abs
SARS-CoV-2 (COVID-19) Spike S2 antibody [GT745] WB, ELISA, Sandwich ELISA, IHC-P (cell pellet) GTX636037  
SARS-CoV-2 (COVID-19) Spike S2 antibody [GT8110] WB, ELISA, Sandwich ELISA, IHC-P (cell pellet) GTX636038  
SARS-CoV-2 (COVID-19) Spike RBD antibody [GT5449] WB, ELISA, Sandwich ELISA GTX636042