SARS-CoV-2 (COVID-19) Antibodies and Reagents

GeneTex has augmented its extensive catalog of SARS-CoV-2-related antibodies and reagents with the production of recombinant antibodies targeting the viral spike and nucleocapsid proteins. The spike S1 recombinant antibodies demonstrate specificity for SARS-CoV-2 S1 with no cross-reactivity to SARS-CoV or MERS-CoV spike proteins. Clones [HL1] and [HL6] in particular exhibit high affinity for mammalian-expressed SARS-CoV-2 spike protein with EC50 values of 0.117 nM and 0.251 nM, respectively. Importantly, both antibodies perform exceptionally well when paired with GeneTex’s mouse monoclonal SARS-CoV antibody [1A9] for sandwich ELISA assays. Though there can be high homology among various regions of different coronavirus nucleocapsid proteins, GeneTex’s SARS-CoV-2 nucleocapsid antibody [HL344] is specific for SARS-CoV-2 with no cross-reactivity to MERS-CoV or SARS-CoV nucleocapsid. All eight recombinant nucleocapsid clones display high affinity for mammalian-expressed full-length SARS-CoV-2 nucleocapsid protein (EC50 values ranging from 0.006-0.074 nM), among which a SARS-CoV-2 nucleocapsid recombinant antibody pair (GTX635685; GTX635712) designed for sandwich ELISA (sELISA; GTX500042) reaching a superior sensitivity of ~3 pg/mL of recombinant nucleocapsid protein.

GeneTex is now developing reagents for the new coronavirus variant, VUI-202012/01, defined by a set of seventeen mutations with many of those in the viral spike protein. This new strain is significantly more transmissible.

Full Listing of Products for SARS-CoV-2 (COVID-19) Research

Anti-Spike Antibodies

Product Name Clonality EC50 Applications Cat. No. Spike S1 antibody [HL1] Rb recAb 117 pM WB, ICC/IF, ELISA, Sandwich ELISA GTX635656 Spike S1 antibody [HL6] Rb recAb 251 pM WB, ICC/IF, IHC-P (cell pellet), ELISA, Sandwich ELISA, FACS, IHC-P GTX635654 Spike S1 antibody [HL134] Rb recAb 552 pM WB, ICC/IF, IHC-P (cell pellet), ELISA GTX635671 Spike S1 antibody [HL263] Rb recAb 365 pM WB, ICC/IF, IHC-P (cell pellet), ELISA, Sandwich ELISA GTX635672 Spike S1 antibody [HL13402] Rb recAb   WB, ICC/IF, ELISA, Sandwich ELISA GTX635713 Spike S1 antibody [GT263] Ms mAb   WB, ICC/IF, IHC-P (cell pellet), ELISA, Sandwich ELISA GTX635708   Spike S1 antibody Rb pAb   WB, ICC/IF, ELISA GTX135384 Spike RBD antibody [HL1003] Rb recAb 7.49 pM ICC/IF, ELISA, Neutralizing/Inhibition,Sandwich ELISA GTX635792 Spike RBD antibody [HL1003-HU] Hu recAb   Neutralizing/Inhibition GTX635866 Spike RBD antibody [HL1004] Rb recAb 25.13 pM ICC/IF, ELISA, Sandwich ELISA, Neutralizing/Inhibition GTX635793 Spike RBD antibody [HL1014] Rb recAb 28.77 pM ICC/IF, ELISA, Sandwich ELISA GTX635807 Spike RBD antibody [HL257] Rb recAb   WB, ICC/IF, IHC-P (cell pellet), ELISA GTX635692   Spike RBD antibody Rb pAb   WB, FACS, Neutralizing/Inhibition, ICC/IF, ELISA, IHC-P (cell pellet) GTX135709   Spike RBD antibody Rb pAb   WB, ICC/IF GTX135385 Spike S2 antibody [HL237] Rb recAb   WB, ICC/IF, IHC-P (cell pellet), ELISA, Sandwich ELISA GTX635693   Spike S2 / S2' antibody Rb pAb   WB, ICC/IF, ELISA, Sandwich ELISA GTX135386 Spike antibody [1A9] Ms mAb   WB, ICC/IF, IHC-P, IHC-P (cell pellet), FACS, IP, ELISA, Sandwich ELISA GTX632604   Spike antibody Rb pAb   WB, ICC/IF, IHC-P (cell pellet), ELISA, Sandwich ELISA GTX135356   Spike antibody Rb pAb   WB, ICC/IF, ELISA, Sandwich ELISA GTX135360 Spike antibody [CR3022] Hu recAb   ELISA, Neutralizing/Inhibition GTX01555 Spike antibody [CR3022-RB] Rb recAb   ELISA, Neutralizing/Inhibition GTX01556

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Anti-Nucleocapsid Antibodies

Product Name Clonality EC50 Applications Cat. No. Nucleocapsid antibody [HL455-MS] Ms mAb   WB, ICC/IF, IHC-P (cell pellet), ELISA, Sandwich ELISA GTX635712 Nucleocapsid antibody [HL5410] Rb recAb 9 pM WB, ICC/IF, IHC-P (cell pellet), ELISA, Sandwich ELISA GTX635685 Nucleocapsid antibody [HL5410] (Gold) Rb recAb   Immunoassay GTX635685-17 Nucleocapsid antibody [HL5511] Rb recAb 14 pM WB, ICC/IF, IHC-P (cell pellet), ELISA, Sandwich ELISA GTX635689 Nucleocapsid antibody [HL146] Rb recAb 50 pM WB, ICC/IF, ELISA, Sandwich ELISA GTX635680 Nucleocapsid antibody [HL249] Rb recAb 16 pM WB, ICC/IF, IHC-P (cell pellet), ELISA, Sandwich ELISA GTX635678 Nucleocapsid antibody [HL344] Rb recAb 74 pM WB, ICC/IF, IHC-P, IHC-P (cell pellet), IHC-Fr, ELISA, Sandwich ELISA GTX635679 Nucleocapsid antibody [HL448] Rb recAb 11 pM WB, ICC/IF, IHC-P, IHC-P (cell pellet), ELISA, Sandwich ELISA GTX635686 Nucleocapsid antibody [HL448] (HRP) Rb recAb   ELISA, Sandwich ELISA, WB GTX635686-01 Nucleocapsid antibody [HL453] Rb recAb 6 pM WB, ICC/IF, IHC-P (cell pellet), ELISA GTX635687 Nucleocapsid antibody [HL455] Rb recAb 6 pM WB, ICC/IF, IHC-P (cell pellet), ELISA, Sandwich ELISA GTX635688   Nucleocapsid antibody [GT113] Ms mAb   WB GTX635808 Nucleocapsid antibody [6H3] Ms mAb   WB, ICC/IF, IHC-P (cell pellet), IP, ELISA, Sandwich ELISA GTX632269 Nucleocapsid antibody Rb pAb   WB, ICC/IF, IHC-P, IHC-P (cell pellet), FACS, IP, ELISA, Sandwich ELISA GTX135357   Nucleocapsid antibody Rb pAb   WB, ICC/IF, IHC-P (cell pellet), ELISA, Sandwich ELISA GTX135361

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Other SARS-CoV-2 Antibodies

Product Name Clonality Applications Cat. No.   RdRp (nsp12) antibody Rb pAb WB, ICC/IF GTX135467   RdRp (nsp12) antibody Rb pAb WB GTX135469   ORF8 antibody Rb pAb WB, ICC/IF GTX135591   nsp1 antibody Rb pAb WB GTX135586   nsp1 antibody Rb pAb WB, ICC/IF GTX135612   PLpro (nsp3) antibody Rb pAb WB, ICC/IF GTX135589   PLpro (nsp3) antibody Rb pAb WB, ICC/IF GTX135614 ORF7a antibody [3C9] Ms mAb WB, ICC/IF GTX632602 nsp8 antibody [5A10] Ms mAb WB, ICC/IF, FACS GTX632696   nsp8 antibody Rb pAb WB GTX135730   Nsp11 antibody Rb pAb WB GTX135742

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ELISA Antibody Pairs

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SARS-CoV-2 Nucleocapsid ELISA Kit

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Lateral Flow Assay (Recommended Antibody Pairs)

Antibody Pair   Product Name Clonality EC50 Cat. No. Pair I Nucleocapsid antibody [HL5511] Rb recAb 14 pM GTX635689 Nucleocapsid antibody [HL448] Rb recAb 11 pM GTX635686 Pair II Nucleocapsid antibody [HL5410] Rb recAb 9 pM GTX635685 Nucleocapsid antibody [HL455] Rb recAb 6 pM GTX635688

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Recombinant Proteins

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Cell Pellet Blocks

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Overexpression Lysates

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Products for Host Cell Entry Research

Product Name Clonality or Expression System Applications Cat. No.    ACE2 antibody [N1N2], N-term Rb pAb WB, ICC/IF, IHC-P, FACS, ELISA GTX101395 ACE2 antibody [SN0754] Rb recAb WB, ICC/IF, IHC-P GTX01160   ACE2 antibody Rb pAb WB, ICC/IF, IHC-P, ELISA GTX15349   Human ACE2(ECD) protein, mouse IgG Fc tag HEK 293 WB, ELISA, Functional Assay GTX135683-pro   Human ACE2 protein, His and Avi tag HEK 293 ELISA GTX01550-pro   TMPRSS2 antibody [N2C3] Rb pAb WB, IHC-P GTX100743   Camostat mesylate   TMPRSS2 inhibitor GTX01523

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Featured Antibodies

Spike S1 antibody [HL6] (GTX635654)

Nucleocapsid antibody [HL5410] (Gold) (GTX635685-17)

Spike S1 antibody [HL263] (GTX635672)

Nucleocapsid antibody [HL448] (GTX635686)

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Highlighted ELISA Pairs

Nucleocapsid ELISA Pair [HL5410 / HL455-MS] (GTX500042)

Nucleocapsid ELISA Pair [HL5511 / HL448] (GTX500045)

Spike ELISA Pair [1A9 / HL6] (GTX500040)

Spike ELISA Pair [1A9 / HL263] (GTX500041)

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Highlighted Proteins

Spike S2 (ECD) protein, mouse IgG Fc tag (GTX135684-pro)

Spike RBD protein, His tag (active) (GTX01546-pro)

Nucleocapsid protein,His tag (Gold) (GTX135746-pro)

Envelope protein, His and Avi tag (GTX01547-pro)

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