SARS-CoV-2 (COVID-19) Antibodies and Reagents

06-May-2021

GeneTex offers a comprehensive catalog of outstanding antibodies and reagents for SARS-CoV-2 research. Antibody clones [HL13402], [HL1014], and [1A9] in particular exhibit high affinity for all SARS-CoV-2 spike proteins, including those of the 501Y.V1 (B.1.1.7) and 501Y.V2 (B.1.351) variants. Recombinant rabbit clone [HL13402], raised against the viral spike S1 subunit, performs exceptionally well when paired with the widely published mouse clone [1A9] (against the S2 subunit) for sandwich ELISA assays. GeneTex’s large collection of spike antibodies virtually guarantees that you will find a reagent appropriate for your desired application. Although there is high homology between different regions of the nucleocapsid proteins from MERS-CoV, SARS-CoV, and SARS-CoV-2, several of GeneTex’s SARS-CoV-2 nucleocapsid antibodies detect only SARS-CoV-2. All recombinant nucleocapsid clones display high affinity for human cell-expressed full-length SARS-CoV-2 nucleocapsid protein, with EC50 values ranging from 0.006-0.074 nM. The SARS-CoV-2 nucleocapsid recombinant antibody pair (GTX635689 and GTX635686) demonstrates superior sensitivity for sandwich ELISA and lateral flow assays. As with the spike antibody catalog, GeneTex’s extensive nucleocapsid antibody listing includes products ideal for all common applications.

GeneTex is using indirect ELISA assays to rigorously test the affinities of several of its spike antibodies for recombinant variant spike (ECD) proteins. Please see the chart below documenting current progress.

Targeted Region of Spike	Antibody Cat. No.	WIV04/2019 (reference sequence)	501Y.V1	501Y.V2	501Y.V3
RBD	GTX635807	●	●	●	●
RBD	GTX635792	●	●	○	○
RBD	GTX635793	●	●	○	●
S1	GTX635713	●	●	●	●
S1	GTX635708	X	X	○	X
S2	GTX632604	●	●	●	○
S2	GTX635693	○	●	●	X

●		Strong reaction. Defined as OD₄₅₀ value >1 when 1μg/ml protein was used.
○		Weak reaction. Defined as OD₄₅₀ value >1 when 4μg/ml protein was used.
X		No reaction. Defined as OD₄₅₀ value <1 when 4μg/ml protein was used.

Full Listing of Products for SARS-CoV-2 (COVID-19) Research

Anti-Spike Antibodies

Product Name	Clonality	EC50	Applications	Cat. No.
Spike S1 antibody [HL1]	Rb recAb	117 pM	WB, ICC/IF, ELISA, Sandwich ELISA	GTX635656
Spike S1 antibody [HL6]	Rb recAb	251 pM	WB, ICC/IF, IHC-P (cell pellet), ELISA, Sandwich ELISA, FACS, IHC-P	GTX635654
Spike S1 antibody [HL134]	Rb recAb	552 pM	WB, ICC/IF, IHC-P (cell pellet), ELISA	GTX635671
Spike S1 antibody [HL263]	Rb recAb	365 pM	WB, ICC/IF, IHC-P (cell pellet), ELISA, Sandwich ELISA	GTX635672
Spike S1 antibody [HL13402]	Rb recAb		WB, ICC/IF, ELISA, Sandwich ELISA	GTX635713
Spike S1 antibody [GT263]	Ms mAb		WB, ICC/IF, IHC-P (cell pellet), ELISA, Sandwich ELISA	GTX635708
Spike S1 antibody	Rb pAb		WB, ICC/IF, ELISA	GTX135384
Spike RBD antibody [HL1002]	Rb recAb		Neutralizing/Inhibition	GTX635791
Spike RBD antibody [HL1003]	Rb recAb	7.49 pM	ICC/IF, ELISA, Neutralizing/Inhibition,Sandwich ELISA	GTX635792
Spike RBD antibody [HL1003-HU]	Hu recAb		Neutralizing/Inhibition	GTX635866
Spike RBD antibody [HL1004]	Rb recAb	25.13 pM	ICC/IF, ELISA, Sandwich ELISA, Neutralizing/Inhibition	GTX635793
Spike RBD antibody [HL1004] (HRP)	Rb recAb		ELISA, Sandwich ELISA	GTX635793-01
Spike RBD antibody [HL1014]	Rb recAb	28.77 pM	ICC/IF, ELISA, Sandwich ELISA	GTX635807
Spike RBD antibody [HL257]	Rb recAb		WB, ICC/IF, IHC-P, ELISA, IHC-P (cell pellet)	GTX635692
Spike RBD antibody	Rb pAb		WB, FACS, Neutralizing/Inhibition, ICC/IF, ELISA, IHC-P (cell pellet)	GTX135709
Spike RBD antibody	Rb pAb		WB, ICC/IF	GTX135385
Spike S2 antibody [HL237]	Rb recAb		WB, ICC/IF, IHC-P (cell pellet), ELISA, Sandwich ELISA	GTX635693
Spike S2 / S2' antibody	Rb pAb		WB, ICC/IF, ELISA, Sandwich ELISA	GTX135386
Spike antibody [1A9]	Ms mAb		WB, ICC/IF, IHC-P, IHC-P (cell pellet), FACS, IP, ELISA, Sandwich ELISA	GTX632604
Spike antibody	Rb pAb		WB, ICC/IF, IHC-P (cell pellet), ELISA, Sandwich ELISA	GTX135356
Spike antibody	Rb pAb		WB, ICC/IF, ELISA, Sandwich ELISA	GTX135360
Spike antibody [CR3022]	Hu recAb		ELISA, Neutralizing/Inhibition	GTX01555
Spike antibody [CR3022-RB]	Rb recAb		ELISA, Neutralizing/Inhibition	GTX01556

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Anti-Nucleocapsid Antibodies

Product Name	Clonality	EC50	Applications	Cat. No.
Nucleocapsid antibody [HL455-MS]	Ms mAb		WB, ICC/IF, IHC-P (cell pellet), ELISA, Sandwich ELISA	GTX635712
Nucleocapsid antibody [HL5410]	Rb recAb	9 pM	WB, ICC/IF, IHC-P (cell pellet), ELISA, Sandwich ELISA	GTX635685
Nucleocapsid antibody [HL5410] (Gold)	Rb recAb		Immunoassay	GTX635685-17
Nucleocapsid antibody [HL5511]	Rb recAb	14 pM	WB, ICC/IF, IHC-P (cell pellet), ELISA, Sandwich ELISA	GTX635689
Nucleocapsid antibody [HL146]	Rb recAb	50 pM	WB, ICC/IF, ELISA, Sandwich ELISA	GTX635680
Nucleocapsid antibody [HL249]	Rb recAb	16 pM	WB, ICC/IF, IHC-P (cell pellet), ELISA, Sandwich ELISA	GTX635678
Nucleocapsid antibody [HL344]	Rb recAb	74 pM	WB, ICC/IF, IHC-P, IHC-P (cell pellet), IHC-Fr, ELISA, Sandwich ELISA	GTX635679
Nucleocapsid antibody [HL448]	Rb recAb	11 pM	WB, ICC/IF, IHC-P, IHC-P (cell pellet), ELISA, Sandwich ELISA	GTX635686
Nucleocapsid antibody [HL448] (HRP)	Rb recAb		ELISA, Sandwich ELISA, WB	GTX635686-01
Nucleocapsid antibody [HL453]	Rb recAb	6 pM	WB, ICC/IF, IHC-P (cell pellet), ELISA	GTX635687
Nucleocapsid antibody [HL455]	Rb recAb	6 pM	WB, ICC/IF, IHC-P (cell pellet), ELISA, Sandwich ELISA	GTX635688
Nucleocapsid antibody [GT113]	Ms mAb		WB	GTX635808
Nucleocapsid antibody [6H3]	Ms mAb		WB, ICC/IF, IHC-P (cell pellet), IP, ELISA, Sandwich ELISA	GTX632269
Nucleocapsid antibody	Rb pAb		WB, ICC/IF, IHC-P, IHC-P (cell pellet), FACS, IP, ELISA, Sandwich ELISA	GTX135357
Nucleocapsid antibody	Rb pAb		WB, ICC/IF, IHC-P (cell pellet), ELISA, Sandwich ELISA	GTX135361

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Other SARS-CoV-2 Antibodies

Product Name	Clonality	Applications	Cat. No.
RdRp (nsp12) antibody	Rb pAb	WB, ICC/IF	GTX135467
RdRp (nsp12) antibody	Rb pAb	WB	GTX135469
3CLpro (nsp5) antibody	Rb pAb	WB	GTX135470
ORF8 antibody	Rb pAb	WB, ICC/IF	GTX135591
nsp1 antibody	Rb pAb	WB	GTX135586
nsp1 antibody	Rb pAb	WB, ICC/IF	GTX135612
nsp2 antibody	Rb pAb	WB	GTX135717
PLpro (nsp3) antibody	Rb pAb	WB, ICC/IF	GTX135589
PLpro (nsp3) antibody	Rb pAb	WB, ICC/IF	GTX135614
ORF7a antibody [3C9]	Ms mAb	WB, ICC/IF	GTX632602
nsp8 antibody [5A10]	Ms mAb	WB, ICC/IF, FACS	GTX632696
nsp8 antibody	Rb pAb	WB	GTX135729
nsp8 antibody	Rb pAb	WB	GTX135730
nsp9 antibody	Rb pAb	WB	GTX135731
nsp9 antibody	Rb pAb	WB	GTX135732
nsp10 antibody	Rb pAb	WB	GTX135733
nsp10 antibody	Rb pAb	WB	GTX135734
nsp15 antibody	Rb pAb	WB	GTX135737
nsp15 antibody	Rb pAb	WB	GTX135738
nsp11 antibody	Rb pAb	WB	GTX135742

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Binding / Neutralization Assay Panel

Product Name	Applications	Cat. No.
RBD ACE2 Binding Activity / Neutralization Assay Panel	Neutralizing/Inhibition	GTX300122

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ELISA Antibody Pairs

Product Name	Sensitivity	Cat. No.
Spike RBD ELISA Pair [HL1014 / HL1003]		GTX500046
Spike RBD ELISA pair [HL1014/HL1004]		GTX500047
Spike ELISA Pair [1A9 / HL263]		GTX500041
Spike ELISA Pair [1A9 / HL13402]		GTX500043
Nucleocapsid ELISA Pair [HL5511 / HL448]	39.06 pg/mL	GTX500045
Nucleocapsid ELISA Pair [HL5410 / HL455-MS]	2.68 pg/mL	GTX500042

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SARS-CoV-2 Nucleocapsid ELISA Kit

Product Name	Sensitivity	Applications	Cat. No.
Nucleocapsid Protein Sandwich ELISA Kit	18 pg/ml	ELISA, Sandwich ELISA	GTX535824

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Lateral Flow Assay (Recommended Antibody Pairs)

Antibody Pair	Product Name	Clonality	EC50	Cat. No.
Pair I	Nucleocapsid antibody [HL5511]	Rb recAb	14 pM	GTX635689
Pair I	Nucleocapsid antibody [HL448]	Rb recAb	11 pM	GTX635686
Pair II	Nucleocapsid antibody [HL5410]	Rb recAb	9 pM	GTX635685
Pair II	Nucleocapsid antibody [HL455]	Rb recAb	6 pM	GTX635688

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Recombinant Proteins

Product Name	Expression System	Applications	Cat. No.
Spike (ECD) protein, His tag (active)	HEK293	Functional Assay	GTX02774-pro
Spike (ECD) protein, His tag (active)	HEK293	ELISA, Functional Assay	GTX135972-pro
Spike (D614G Mutant) protein (ECD), His tag (active)	HEK293		GTX02575-pro
Spike (del69-70,del144,N501Y,A570D,D614G,...) (ECD) Protein, His tag (active)	HEK293		GTX136059-pro
Spike (L18F,..., K417T, E484K, N501Y, D614G,...,V987P)(ECD), His tag (active)	HEK293		GTX136091-pro
Spike S1 (del69-70, del44, N501Y, A570D, D614G...) Protein, His tag (active)	HEK293		GTX136085-pro
Spike S1 (L18F..., R190S, K417T, E484K, N501Y, D614G, H655Y), His tag (active)	HEK293		GTX136094-pro
Spike S1 (L18F, D80A,..., R246I, K417N, E484K, N501Y, D614G), His tag (active)	HEK293		GTX136095-pro
Spike S1 protein, His tag (active)	HEK293	ELISA, Functional Assay	GTX01554-pro
Spike S1 protein, His and Avi tag (active)	HEK293	ELISA, Functional Assay	GTX01548-pro
Spike S1 protein, His tag (active)	HEK293	WB, ELISA, Functional Assay, Sandwich ELISA	GTX135817-pro
Spike S2 (ECD) protein, mouse IgG Fc tag	HEK293	WB, ELISA, Sandwich ELISA	GTX135684-pro
Spike S2 (ECD) protein, human IgG Fc tag	HEK293		GTX01559-pro
Spike S2 (T716I, S982A, D1118H Mutant) (ECD) protein, His tag	HEK293	WB	GTX136023-pro
Spike RBD (E484K, N501Y Mutant) protein, His tag (active)	HEK293		GTX136058-pro
Spike RBD (N501Y Mutant) protein, His tag (active)	HEK293	WB, ELISA, Functional Assay	GTX136014-pro
Spike RBD (K417N, E484K, N501Y Mutant) protein, His tag (active)	HEK293	WB, Functional Assay	GTX136022-pro
Spike RBD (K417T, E484K, N501Y Mutant) protein, His tag	HEK293		GTX136043-pro
Spike RBD protein, His tag (active)	HEK293	Functional Assay	GTX136090-pro
Spike RBD protein, His tag (active)	HEK293	Functional Assay	GTX01546-pro
Nucleocapsid protein, His tag	E. coli	ELISA, Sandwich ELISA	GTX135357-pro
Nucleocapsid protein, His tag	HEK293	WB, ELISA, Sandwich ELISA	GTX135592-pro
Nucleocapsid protein,His tag (Gold)	HEK293	Immunoassay	GTX135746-pro
Envelope protein, His and Avi tag	E. coli		GTX01547-pro
Envelope Protein, GST and His Tag	HEK293		GTX01565-pro
3CLpro (nsp5) protein, His tag	E. coli	WB	GTX135648-pro
3CLpro (nsp5) protein, His and Avi tag	E. coli		GTX01557-pro

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Cell Pellet Blocks

Product Name	Applications	Cat. No.
Spike FFPE 293T cell pellet block	IHC-P (cell pellet)	GTX435640
Spike S1 FFPE 293T cell pellet block	IHC-P (cell pellet)	GTX435643
Spike S2 FFPE 293T cell pellet block	IHC-P (cell pellet)	GTX435644
Nucleocapsid FFPE 293T cell pellet block	IHC-P (cell pellet)	GTX435641
Envelope FFPE 293T cell pellet block	IHC-P (cell pellet)	GTX435642
Membrane FFPE 293T cell pellet block	IHC-P (cell pellet)	GTX435645

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Overexpression Lysates

Product Name	Applications	Cat. No.
Spike overexpression 293T whole cell lysate	WB, ELISA	GTX535664
Spike S1 overexpression 293T whole cell lysate	WB	GTX535663
Nucleocapsid overexpression 293T whole cell lysate	WB, ELISA	GTX535665

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Products for Host Cell Entry Research

Product Name	Clonality or Expression System	Applications	Cat. No.
ACE2 antibody [GT19410]	Ms mAb	IHC-P, ELISA	GTX635897
ACE2 antibody [N1N2], N-term	Rb pAb	WB, ICC/IF, IHC-P, FACS, ELISA	GTX101395
ACE2 antibody [SN0754]	Rb recAb	WB, ICC/IF, IHC-P	GTX01160
ACE2 antibody	Rb pAb	WB, ICC/IF, IHC-P, ELISA	GTX15349
Human ACE2(ECD) protein, mouse IgG Fc tag	HEK 293	WB, ELISA, Functional Assay	GTX135683-pro
Human ACE2 protein, His and Avi tag	HEK 293	ELISA	GTX01550-pro
TMPRSS2 antibody [N2C3]	Rb pAb	WB, IHC-P	GTX100743
Camostat mesylate		TMPRSS2 inhibitor	GTX01523