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SARS-CoV-2 (COVID-19) Antibodies and Reagents

14-Sep-2020
spike protein / Nucleocapsid protein

 

GeneTex has augmented its extensive catalog of SARS-CoV-2-related antibodies and reagents with the production of recombinant antibodies targeting the viral spike and nucleocapsid proteins. The spike S1 recombinant antibodies demonstrate specificity for SARS-CoV-2 S1 with no cross-reactivity to SARS-CoV or MERS-CoV spike proteins. Clones [HL1] and [HL6] in particular exhibit high affinity for mammalian-expressed SARS-CoV-2 spike protein with EC50 values of 0.117 nM and 0.251 nM, respectively. Importantly, both antibodies perform exceptionally well when paired with GeneTex’s mouse monoclonal SARS-CoV antibody [1A9] for sandwich ELISA assays. Though there can be high homology among various regions of different coronavirus nucleocapsid proteins, GeneTex’s SARS-CoV-2 nucleocapsid antibody [HL344] is specific for SARS-CoV-2 with no cross-reactivity to MERS-CoV or SARS-CoV nucleocapsid. All eight recombinant nucleocapsid clones display high affinity for mammalian-expressed full-length SARS-CoV-2 nucleocapsid protein (EC50 values ranging from 0.006-0.074 nM), among which a SARS-CoV-2 nucleocapsid recombinant antibody pair (GTX635685; GTX635712) designed for sandwich ELISA (sELISA; GTX500042) reaching a superior sensitivity of ~3 pg/mL of recombinant nucleocapsid protein.

 

Full Listing of Products for SARS-CoV-2 (COVID-19) Research

Anti-Spike antibodies

Recombinant Citation-Support Protein Overexpression 

  Product Name Clonality EC50 or Sensitivity Applications Cat. No.
Spike S1 antibody [HL1] Rb recAb 0.117 nM WB, ICC/IF, ELISA, Sandwich ELISA GTX635656
Spike S1 antibody [HL6] Rb recAb 0.552 nM WB, ICC/IF, IHC-P (cell pellet), ELISA, Sandwich ELISA, FACS, IHC-P GTX635654
Spike S1 antibody [HL134] Rb recAb 0.251 nM WB, ICC/IF, IHC-P (cell pellet), ELISA GTX635671
Spike S1 antibody [HL263] Rb recAb 0.672 nM WB, ICC/IF, IHC-P (cell pellet), ELISA, Sandwich ELISA GTX635672
Spike S1 antibody [HL13402] Rb recAb   WB, ICC/IF, ELISA, Sandwich ELISA GTX635713
Spike S1 antibody [GT263] Ms mAb   WB, ICC/IF, IHC-P (cell pellet), ELISA, Sandwich ELISA GTX635708
  Spike S1 antibody Rb pAb   WB, ICC/IF, ELISA GTX135384
Spike RBD antibody [HL257] Rb recAb   WB, ICC/IF, IHC-P (cell pellet), ELISA GTX635692
  Spike RBD antibody Rb pAb   WB, FACS, Neutralizing/Inhibition, ICC/IF, ELISA GTX135709
  Spike RBD antibody Rb pAb   WB, ICC/IF GTX135385
Spike S2 antibody [HL237] Rb recAb   WB, ICC/IF, IHC-P (cell pellet), ELISA, Sandwich ELISA GTX635693
  Spike S2 / S2' antibody Rb pAb   WB, ICC/IF, ELISA, Sandwich ELISA GTX135386
Spike antibody [1A9] Ms mAb   WB, ICC/IF, IHC-P, IHC-P (cell pellet), FACS, IP, ELISA, Sandwich ELISA GTX632604
  Spike antibody Rb pAb   WB, ICC/IF, IHC-P (cell pellet), ELISA, Sandwich ELISA GTX135356
  Spike antibody Rb pAb   WB, ICC/IF, ELISA, Sandwich ELISA GTX135360
Spike antibody [CR3022] Hu recAb   ELISA, Neutralizing/Inhibition GTX01555
Spike antibody [CR3022-RB] Rb recAb   ELISA, Neutralizing/Inhibition GTX01556

 

Anti-Nucleocapsid antibodies

Recombinant Citation-Support Protein Overexpression 

  Product Name Clonality EC50 or Sensitivity Applications Cat. No.
Nucleocapsid antibody [HL455-MS] Ms mAb 2.68 pg/mL WB, ICC/IF, IHC-P (cell pellet), ELISA, Sandwich ELISA GTX635712
Nucleocapsid antibody [HL5410] Rb recAb 0.009 nM WB, ICC/IF, IHC-P (cell pellet), ELISA, Sandwich ELISA GTX635685
Nucleocapsid antibody [HL5410] (Gold) Rb recAb   Immunoassay GTX635685-17
Nucleocapsid antibody [HL5511] Rb recAb 0.014 nM WB, ICC/IF, IHC-P (cell pellet), ELISA, Sandwich ELISA GTX635689
Nucleocapsid antibody [HL146] Rb recAb 0.05 nM WB, ICC/IF, ELISA, Sandwich ELISA GTX635680
Nucleocapsid antibody [HL249] Rb recAb 0.016 nM WB, ICC/IF, IHC-P (cell pellet), ELISA, Sandwich ELISA GTX635678
Nucleocapsid antibody [HL344] Rb recAb 0.074 nM WB, ICC/IF, IHC-P, IHC-P (cell pellet), IHC-Fr, ELISA, Sandwich ELISA GTX635679
Nucleocapsid antibody [HL448] Rb recAb 0.011 nM WB, ICC/IF, IHC-P, IHC-P (cell pellet), ELISA, Sandwich ELISA GTX635686
Nucleocapsid antibody [HL448] (HRP) Rb recAb 39.06 pg/mL ELISA, Sandwich ELISA GTX635686-01
Nucleocapsid antibody [HL453] Rb recAb 0.006 nM WB, ICC/IF, IHC-P (cell pellet), ELISA GTX635687
Nucleocapsid antibody [HL455] Rb recAb 0.006 nM WB, ICC/IF, IHC-P (cell pellet), ELISA, Sandwich ELISA GTX635688
Nucleocapsid antibody [6H3] Ms mAb   WB, ICC/IF, IHC-P (cell pellet), IP, ELISA, Sandwich ELISA GTX632269
Nucleocapsid antibody Rb pAb   WB, ICC/IF, IHC-P, IHC-P (cell pellet), IHC-Fr, FACS, IP, ELISA, Sandwich ELISA GTX135357
  Nucleocapsid antibody Rb pAb   WB, ICC/IF, IHC-P (cell pellet), ELISA, Sandwich ELISA GTX135361

 

Other Antibodies

Citation-Support  

  Product Name Clonality Applications Cat. No.
  RdRp (nsp12) antibody Rb pAb WB, ICC/IF GTX135467
  RdRp (nsp12) antibody Rb pAb WB GTX135469
  ORF8 antibody Rb pAb WB, ICC/IF GTX135591
  nsp1 antibody Rb pAb WB, ICC/IF GTX135612
  PLpro (nsp3) antibody Rb pAb WB, ICC/IF GTX135589
  PLpro (nsp3) antibody Rb pAb WB, ICC/IF GTX135614
ORF7a antibody [3C9] Ms mAb WB, ICC/IF GTX632602
NSP8 antibody [5A10] Ms mAb WB, ICC/IF, FACS GTX632696

 

ELISA Pairs

Product Name Cat. No.
Spike ELISA Pair - [1A9 / HL263] GTX500041
Spike ELISA Pair - [1A9 / HL6] GTX500040
Spike ELISA Pair [1A9 / HL13402] GTX500043
Nucleocapsid ELISA Pair [HL5511 / HL448] GTX500045
Nucleocapsid ELISA Pair [HL5410 / HL455-MS] GTX500042

 

Lateral Flow Recommended Pairs  

Recombinant 

Pair   Product Name Clonality EC50 Cat. No.
Pair I Nucleocapsid antibody [HL5511] Rb recAb 0.014 nM GTX635689
Nucleocapsid antibody [HL448] Rb recAb 0.011 nM GTX635686
Pair II Nucleocapsid antibody [HL5410] Rb recAb 0.009 nM GTX635685
Nucleocapsid antibody [HL455] Rb recAb 0.006 nM GTX635688

 

Recombinant Proteins

Product Name Expression System Applications Cat. No.
Spike S1 protein, His tag (active) HEK293 ELISA GTX01554-pro
Spike S1 protein, His and Avi tag (active) HEK293   GTX01548-pro
Spike S2 (ECD) protein, mouse IgG Fc tag HEK293   GTX135684-pro
Spike S2 (ECD) protein, human IgG Fc tag HEK293   GTX01559-pro
Spike RBD protein, His tag (active) HEK293   GTX01546-pro
Nucleocapsid protein, His tag E. coli ELISA GTX135357-pro
Nucleocapsid protein, His tag HEK293 WB, ELISA GTX135592-pro
Nucleocapsid protein,His tag (Gold) HEK293 Immunoassay GTX135746-pro
Envelope protein, His and Avi tag E. coli   GTX01547-pro
Envelope Protein, GST and His Tag HEK293   GTX01565-pro
3CLpro (nsp5) protein, His tag E. coli   GTX135648-pro
3CLpro (nsp5) protein, His and Avi tag E. coli   GTX01557-pro

 

Cell Pellet Blocks

Product Name Applications Cat. No.
Spike FFPE 293T cell pellet block IHC-P (cell pellet) GTX435640
Spike S1 FFPE 293T cell pellet block IHC-P (cell pellet) GTX435643
Spike S2 FFPE 293T cell pellet block IHC-P (cell pellet) GTX435644
Nucleocapsid FFPE 293T cell pellet block IHC-P (cell pellet) GTX435641
Envelope FFPE 293T cell pellet block IHC-P (cell pellet) GTX435642
Membrane FFPE 293T cell pellet block IHC-P (cell pellet) GTX435645

 

Overexpression Lysates

Product Name Applications Cat. No.
Spike overexpression 293T whole cell lysate WB, ELISA GTX535664
Spike S1 overexpression 293T whole cell lysate WB GTX535663
Nucleocapsid overexpression 293T whole cell lysate WB, ELISA GTX535665
Spike overexpression 293T whole cell lysate WB GTX535668

 

Products for Host Cell Entry Research

Recombinant Citation-Support KOKD-Validation 

  Product Name Clonality or Expression System Applications Cat. No.
  ACE2 antibody [N1N2], N-term Rb pAb WB, ICC/IF, IHC-P, FACS, ELISA GTX101395
ACE2 antibody [SN0754] Rec Rb pAb WB, ICC/IF, IHC-P GTX01160
  ACE2 antibody Rb pAb WB, ICC/IF, IHC-P, ELISA GTX15349
  Human ACE2(ECD) protein, mouse IgG Fc tag HEK 293 WB, ELISA, Functional Assay GTX135683-pro
  Human ACE2 protein, His and Avi tag HEK 293 ELISA GTX01550-pro
  TMPRSS2 antibody [N2C3] Rb pAb WB, IHC-P GTX100743
  Camostat mesylate   TMPRSS2 inhibitor GTX01523

 

Featured Antibodies

Recombinant
Spike S1 antibody [HL6] (GTX635654)

Spike S1 antibody [HL6] (GTX635654)

Nucleocapsid antibody Nucleocapsid antibody [HL5410] (Gold) (GTX635685-17)

Nucleocapsid antibody [HL5410] (Gold) (GTX635685-17)

Spike S1 antibody [HL263] (GTX635672)

Spike S1 antibody [HL263] (GTX635672)

Nucleocapsid antibody [HL448] (GTX635686)

Nucleocapsid antibody [HL448] (GTX635686)

 

Highlighted ELISA Pairs

Nucleocapsid ELISA Pair [HL5410 / HL455-MS] (GTX500042)

Nucleocapsid ELISA Pair [HL5410 / HL455-MS] (GTX500042)

Nucleocapsid ELISA Pair [HL5511 / HL448] (GTX500045)

Nucleocapsid ELISA Pair [HL5511 / HL448] (GTX500045)

Spike ELISA Pair [1A9 / HL6] (GTX500040)

Spike ELISA Pair [1A9 / HL6] (GTX500040)

Spike ELISA Pair [1A9 / HL263] (GTX500041)

Spike ELISA Pair [1A9 / HL263] (GTX500041)

 

Highlighted Proteins

Spike S2 (ECD) protein, mouse IgG Fc tag (GTX135684-pro)

Spike S2 (ECD) protein, mouse IgG Fc tag (GTX135684-pro)

Spike RBD protein, His tag (active) (GTX01546-pro)

Spike RBD protein, His tag (active) (GTX01546-pro)

Nucleocapsid protein,His tag (Gold) (GTX135746-pro)

Nucleocapsid protein,His tag (Gold) (GTX135746-pro)

Envelope protein, His and Avi tag (GTX01547-pro)

Envelope protein, His and Avi tag (GTX01547-pro)